Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1191886..1192540 Replicon chromosome
Accession NZ_CP010121
Organism Escherichia coli strain C4

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag RG27_RS06150 Protein ID WP_000244781.1
Coordinates 1192133..1192540 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag RG27_RS06145 Protein ID WP_000354046.1
Coordinates 1191886..1192152 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG27_RS06125 1187974..1189407 - 1434 WP_001314995.1 6-phospho-beta-glucosidase BglA -
RG27_RS06130 1189452..1189763 + 312 WP_001182952.1 N(4)-acetylcytidine aminohydrolase -
RG27_RS06135 1189927..1190586 + 660 WP_000250269.1 hemolysin III family protein -
RG27_RS06140 1190663..1191643 - 981 WP_000886089.1 tRNA-modifying protein YgfZ -
RG27_RS06145 1191886..1192152 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
RG27_RS06150 1192133..1192540 + 408 WP_000244781.1 protein YgfX Toxin
RG27_RS06155 1192580..1193101 - 522 WP_001055865.1 flavodoxin FldB -
RG27_RS06160 1193213..1194109 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
RG27_RS06165 1194134..1194844 + 711 WP_000715221.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
RG27_RS06170 1194850..1196583 + 1734 WP_000813188.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T50456 WP_000244781.1 NZ_CP010121:1192133-1192540 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T50456 NZ_CP010121:1192133-1192540 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATCTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATCCACGGGTTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT50456 WP_000354046.1 NZ_CP010121:1191886-1192152 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT50456 NZ_CP010121:1191886-1192152 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAACCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References