Detailed information of TA system TA050249

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 2082019..2082676
• Replicon: chromosome
• Accession: NZ_CP010017
• Organism: Burkholderia thailandensis 34

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: BTM_RS11010
• Protein ID: WP_045599895.1
• Coordinates: 2082344..2082676 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BTM_RS33395
• Protein ID: WP_082255747.1
• Coordinates: 2082019..2082351 (-)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTM_RS10985	2077372..2078592	+	1221	WP_025405302.1	ISL3 family transposase	-
BTM_RS10990	2078662..2078949	-	288	Protein_1865	phage portal protein	-
BTM_RS10995	2079029..2079226	+	198	WP_045599893.1	hypothetical protein	-
BTM_RS11000	2079274..2080053	+	780	Protein_1867	site-specific integrase	-
BTM_RS11005	2080363..2081871	-	1509	WP_045599894.1	hypothetical protein	-
BTM_RS33395	2082019..2082351	-	333	WP_082255747.1	helix-turn-helix domain-containing protein	Antitoxin
BTM_RS11010	2082344..2082676	-	333	WP_045599895.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BTM_RS11015	2082718..2083305	-	588	WP_045600852.1	site-specific integrase	-
BTM_RS11020	2083653..2084432	-	780	WP_045599896.1	hypothetical protein	-
BTM_RS11025	2084957..2085190	+	234	WP_045599897.1	hypothetical protein	-
BTM_RS11030	2085204..2086568	-	1365	WP_045599900.1	type II secretory pathway protein	-
BTM_RS11035	2086565..2087491	-	927	WP_045599095.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2054561..2096073	41512

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (52-105)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12787.85 Da
• Isoelectric Point: 10.1361

>T50249 WP_045599895.1 NZ_CP010017:c2082676-2082344 [Burkholderia thailandensis 34]
MKLDWTVVYYNERVKRDVFAFPAGILADYLRLLDLMQEFGADLRMPHSRAMGNGLFELRPKGREGIGRVFYCTHVGQQVV
VLHSFVKKTQETPQHELRIAQARLREVRNG

Nucleotide

• Length: 333 bp

>T50249 NZ_CP010017:c2082676-2082344 [Burkholderia thailandensis 34]
ATGAAACTTGATTGGACGGTCGTCTACTACAACGAACGGGTGAAGCGCGACGTTTTTGCGTTCCCGGCTGGGATTCTTGC
GGACTACCTCCGGCTGTTGGATCTGATGCAGGAGTTCGGTGCGGATCTACGGATGCCGCACTCCCGAGCAATGGGTAACG
GGTTGTTTGAATTGCGTCCTAAGGGTAGGGAAGGTATCGGGCGGGTGTTCTATTGCACGCACGTGGGGCAGCAAGTCGTT
GTGCTGCACTCCTTCGTCAAGAAGACGCAGGAAACGCCGCAACATGAATTGCGGATCGCCCAAGCACGTTTGAGAGAGGT
ACGTAATGGCTAA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 11738.35 Da
• Isoelectric Point: 10.4476

>AT50249 WP_082255747.1 NZ_CP010017:c2082351-2082019 [Burkholderia thailandensis 34]
MAKAATKRVRADGFNPVPHTAEDTARLLAGRNVKAAYDALDDEYTALRAILSARRDAGLTQAQIAERMGTTASAVSRLEA
SLSSEKHSPSFATLRKYAAACGKRLVISFA

Nucleotide

• Length: 333 bp

>AT50249 NZ_CP010017:c2082351-2082019 [Burkholderia thailandensis 34]
ATGGCTAAGGCAGCAACGAAGCGCGTTCGCGCTGATGGATTCAATCCGGTTCCGCATACGGCGGAAGATACGGCACGTCT
GCTCGCCGGTCGCAATGTCAAGGCGGCGTATGACGCCCTGGACGATGAGTACACCGCGTTGCGAGCTATCTTGTCTGCAC
GGCGCGATGCTGGTCTGACGCAGGCTCAGATTGCGGAACGGATGGGTACGACCGCATCGGCGGTTTCGCGGCTTGAGGCT
TCGTTGTCGAGTGAAAAGCATTCACCATCATTTGCGACACTGCGTAAGTATGCGGCTGCATGTGGCAAGCGATTGGTTAT
CTCATTTGCCTAA