Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1221704..1222323 Replicon chromosome
Accession NZ_CP009872
Organism Klebsiella pneumoniae subsp. pneumoniae strain KPNIH30

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KPNIH30_RS06095 Protein ID WP_002892050.1
Coordinates 1221704..1221922 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KPNIH30_RS06100 Protein ID WP_002892066.1
Coordinates 1221949..1222323 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNIH30_RS06060 1217751..1218014 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
KPNIH30_RS06065 1218014..1218154 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KPNIH30_RS06070 1218151..1218849 - 699 WP_002892021.1 GNAT family N-acetyltransferase -
KPNIH30_RS06075 1218950..1220401 + 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
KPNIH30_RS06080 1220376..1220846 - 471 WP_002892026.1 YlaC family protein -
KPNIH30_RS30025 1220867..1221007 + 141 WP_004147370.1 hypothetical protein -
KPNIH30_RS06090 1220979..1221545 - 567 WP_002892030.1 maltose O-acetyltransferase -
KPNIH30_RS06095 1221704..1221922 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
KPNIH30_RS06100 1221949..1222323 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KPNIH30_RS06105 1222809..1225955 - 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit -
KPNIH30_RS06110 1225978..1227171 - 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T50078 WP_002892050.1 NZ_CP009872:c1221922-1221704 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T50078 NZ_CP009872:c1221922-1221704 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT50078 WP_002892066.1 NZ_CP009872:c1222323-1221949 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT50078 NZ_CP009872:c1222323-1221949 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References