Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 4282637..4283153 Replicon chromosome
Accession NZ_CP009850
Organism UNVERIFIED_ORG: Enterobacter cloacae strain ECNIH4

Toxin (Protein)


Gene name relE Uniprot ID A0A2J0PPK2
Locus tag ECNIH4_RS20380 Protein ID WP_014882291.1
Coordinates 4282637..4282921 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V3EXX3
Locus tag ECNIH4_RS20385 Protein ID WP_008501400.1
Coordinates 4282911..4283153 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECNIH4_RS20365 (ECNIH4_20365) 4278947..4279519 + 573 WP_014882294.1 PTS sugar transporter subunit IIA -
ECNIH4_RS20370 (ECNIH4_20370) 4279889..4282027 + 2139 WP_038421312.1 anaerobic ribonucleoside-triphosphate reductase -
ECNIH4_RS20375 (ECNIH4_20375) 4282169..4282633 + 465 WP_014882292.1 anaerobic ribonucleoside-triphosphate reductase-activating protein -
ECNIH4_RS20380 (ECNIH4_20380) 4282637..4282921 - 285 WP_014882291.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ECNIH4_RS20385 (ECNIH4_20385) 4282911..4283153 - 243 WP_008501400.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
ECNIH4_RS20390 (ECNIH4_20390) 4283231..4285141 - 1911 WP_014882290.1 PRD domain-containing protein -
ECNIH4_RS20395 (ECNIH4_20395) 4285161..4286297 - 1137 WP_014882289.1 lactonase family protein -
ECNIH4_RS20400 (ECNIH4_20400) 4286315..4287058 - 744 WP_023331886.1 KDGP aldolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-83)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-69)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10894.69 Da        Isoelectric Point: 10.1988

>T49989 WP_014882291.1 NZ_CP009850:c4282921-4282637 [Enterobacter cloacae]
MTYELVFDPRALKEWSKLGDTVKNQFKKKLADVLVNPRVESARLHGLPDCYKIKLRSQGYRLVYQVQDDVVTVFVIAVGK
REKSAVYHDANKRL

Download         Length: 285 bp

>T49989 NZ_CP009850:c4282921-4282637 [Enterobacter cloacae]
ATGACTTATGAACTGGTATTTGACCCGAGGGCGTTAAAGGAGTGGAGCAAGCTGGGCGATACCGTAAAAAACCAATTTAA
GAAGAAGCTTGCGGACGTGTTGGTGAATCCCAGGGTTGAATCGGCTCGCCTGCATGGCCTGCCTGATTGCTACAAGATAA
AACTGAGATCCCAGGGTTATCGTTTGGTCTATCAGGTTCAGGATGACGTTGTAACGGTATTTGTTATTGCTGTCGGTAAG
AGAGAGAAGTCAGCCGTTTATCATGACGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8972.37 Da        Isoelectric Point: 5.0651

>AT49989 WP_008501400.1 NZ_CP009850:c4283153-4282911 [Enterobacter cloacae]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAETGRMPVKTVTISDSEDALIQTVRERLANPQKGIKVSLDDL

Download         Length: 243 bp

>AT49989 NZ_CP009850:c4283153-4282911 [Enterobacter cloacae]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTCAATATCACGCC
AACGGAAGCTGTGCGTTTACTGTTTCAGTATGTCGCGGAAACGGGACGCATGCCGGTGAAAACGGTCACCATTAGCGACA
GTGAAGATGCACTGATACAGACAGTCCGGGAGCGACTGGCTAATCCGCAAAAGGGAATCAAGGTCAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J0PPK2

Antitoxin

Source ID Structure
AlphaFold DB V3EXX3

References