Detailed information of TA system TA049988

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 3811342..3812057
• Replicon: chromosome
• Accession: NZ_CP009850
• Organism: UNVERIFIED_ORG: Enterobacter cloacae strain ECNIH4

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A8I0UM05
• Locus tag: ECNIH4_RS24395
• Protein ID: WP_023303149.1
• Coordinates: 3811342..3811710 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A8I0UJV2
• Locus tag: ECNIH4_RS18200
• Protein ID: WP_023303148.1
• Coordinates: 3811731..3812057 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECNIH4_RS18165 (ECNIH4_18165)	3806365..3806793	+	429	WP_032638336.1	hypothetical protein	-
ECNIH4_RS18170 (ECNIH4_18170)	3806769..3807332	+	564	Protein_3575	IS3 family transposase	-
ECNIH4_RS18175 (ECNIH4_18175)	3807487..3808350	+	864	WP_001065553.1	GTPase family protein	-
ECNIH4_RS18180 (ECNIH4_18180)	3808442..3809263	+	822	WP_000197389.1	DUF932 domain-containing protein	-
ECNIH4_RS18185 (ECNIH4_18185)	3809480..3810181	+	702	WP_006812588.1	WYL domain-containing protein	-
ECNIH4_RS18190 (ECNIH4_18190)	3810222..3810458	+	237	WP_001144031.1	protein YpjK	-
ECNIH4_RS18195 (ECNIH4_18195)	3810458..3810901	+	444	WP_000824223.1	lipoprotein YafY	-
ECNIH4_RS25355	3810925..3811020	+	96	Protein_3581	hypothetical protein	-
ECNIH4_RS24395	3811342..3811710	-	369	WP_023303149.1	TA system toxin CbtA family protein	Toxin
ECNIH4_RS18200 (ECNIH4_18200)	3811731..3812057	-	327	WP_023303148.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ECNIH4_RS18205 (ECNIH4_18205)	3812070..3812540	-	471	WP_023303147.1	DNA repair protein RadC	-
ECNIH4_RS18210 (ECNIH4_18210)	3812606..3813427	-	822	WP_023303146.1	DUF932 domain-containing protein	-
ECNIH4_RS18220 (ECNIH4_18220)	3814117..3814956	-	840	WP_023303145.1	hypothetical protein	-
ECNIH4_RS18225 (ECNIH4_18225)	3815015..3815449	-	435	WP_023303144.1	hypothetical protein	-
ECNIH4_RS18230 (ECNIH4_18230)	3815758..3817023	-	1266	WP_038421272.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3806790..3807332	542

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (7-107)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13727.91 Da
• Isoelectric Point: 8.2822

>T49988 WP_023303149.1 NZ_CP009850:c3811710-3811342 [Enterobacter cloacae]
MKNLPATISRAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGIILADAVNFLVDKYELVRIDRRGF
SSQGQVPYLTVTDILHARRACGLMKSCSYREVSNIVLSHSRQ

Nucleotide

• Length: 369 bp

>T49988 NZ_CP009850:c3811710-3811342 [Enterobacter cloacae]
ATGAAAAATCTACCTGCTACAATTTCGCGGGCGGCGAAGCCCTGCCTGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTCTGCTTGAACAGCACTATGGTCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTAATTAAGGAACATATTG
ATGCTGGGATTATCCTGGCCGATGCAGTCAACTTCCTGGTAGATAAGTACGAACTGGTTCGCATTGATCGCAGAGGATTT
AGCTCACAGGGACAAGTACCATATTTGACCGTGACAGATATCCTTCATGCCAGAAGAGCATGTGGATTAATGAAAAGTTG
CTCTTATCGGGAAGTAAGTAATATTGTTCTTAGTCATTCGCGGCAGTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12215.04 Da
• Isoelectric Point: 7.0266

>AT49988 WP_023303148.1 NZ_CP009850:c3812057-3811731 [Enterobacter cloacae]
MSLIPAHEWGIKSDIVPRLGVRLVQEGNRLHYLADRASITGKFSDAECLKLDIVFPHFIRQMESMLTTGEMNSRHAHCVT
LYHNGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 327 bp

>AT49988 NZ_CP009850:c3812057-3811731 [Enterobacter cloacae]
ATGTCATTAATACCAGCCCATGAATGGGGGATAAAAAGCGATATTGTTCCACGGCTTGGAGTCAGATTAGTTCAGGAAGG
CAACCGGCTGCACTATCTGGCTGACCGGGCTAGTATCACCGGTAAGTTCAGTGACGCCGAATGCCTGAAGTTGGATATCG
TATTTCCGCATTTTATCCGTCAGATGGAATCGATGCTGACCACTGGTGAAATGAATTCTCGCCATGCCCACTGTGTCACT
CTGTACCACAACGGTTTCACCTGCGAAGCCGATACCCTTGGTAGTTGCGGCTACGTATACATCGCCATTTATCCCACTCA
ACGCTAA