Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3649591..3650211 Replicon chromosome
Accession NZ_CP009850
Organism UNVERIFIED_ORG: Enterobacter cloacae strain ECNIH4

Toxin (Protein)


Gene name Hha Uniprot ID A0A2W0G488
Locus tag ECNIH4_RS17405 Protein ID WP_014882802.1
Coordinates 3649993..3650211 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag ECNIH4_RS17400 Protein ID WP_008499288.1
Coordinates 3649591..3649965 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECNIH4_RS17390 (ECNIH4_17390) 3644717..3645910 + 1194 WP_014882804.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
ECNIH4_RS17395 (ECNIH4_17395) 3645933..3649079 + 3147 WP_014882803.1 multidrug efflux RND transporter permease subunit AcrB -
ECNIH4_RS17400 (ECNIH4_17400) 3649591..3649965 + 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
ECNIH4_RS17405 (ECNIH4_17405) 3649993..3650211 + 219 WP_014882802.1 HHA domain-containing protein Toxin
ECNIH4_RS17410 (ECNIH4_17410) 3650421..3650972 + 552 WP_014882801.1 maltose O-acetyltransferase -
ECNIH4_RS17415 (ECNIH4_17415) 3651091..3651558 + 468 WP_014882800.1 YlaC family protein -
ECNIH4_RS17420 (ECNIH4_17420) 3651530..3652990 - 1461 WP_014882799.1 PLP-dependent aminotransferase family protein -
ECNIH4_RS17425 (ECNIH4_17425) 3653092..3653802 + 711 WP_038421234.1 GNAT family protein -
ECNIH4_RS17430 (ECNIH4_17430) 3653799..3653939 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
ECNIH4_RS17435 (ECNIH4_17435) 3653942..3654202 - 261 WP_010428154.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T49987 WP_014882802.1 NZ_CP009850:3649993-3650211 [Enterobacter cloacae]
MSEKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T49987 NZ_CP009850:3649993-3650211 [Enterobacter cloacae]
ATGTCTGAAAAACCACTCACAAAGGTCGATTATTTGATGCGCTTACGCCGTTGTCAGTCAATTGACACCCTCGAACGCGT
CATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCTGACCATCGCCTCGCTGAGC
TAACGATGAATAAGTTATATGACAAAATTCCCACATCCGTGTGGAAATTTGTACGTTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT49987 WP_008499288.1 NZ_CP009850:3649591-3649965 [Enterobacter cloacae]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT49987 NZ_CP009850:3649591-3649965 [Enterobacter cloacae]
ATGGACGAGTACTCGCCAAAACGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCATGGCTGGGTAAACGATCCAACGTCTGCCATCAATTTACAGTTGAACGAGCTTATAGAGC
ATATCGCTACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGTAGTTACGGCATTAACGCGCAAGATTTGCAAAAATGGCGCAAATCGGGAAACCGACT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCCGTTAGCCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2W0G488

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References