Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3571398..3572047 Replicon chromosome
Accession NZ_CP009850
Organism UNVERIFIED_ORG: Enterobacter cloacae strain ECNIH4

Toxin (Protein)


Gene name higB Uniprot ID A0A2J0PLQ3
Locus tag ECNIH4_RS17055 Protein ID WP_014882864.1
Coordinates 3571398..3571760 (+) Length 121 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2W0GCS7
Locus tag ECNIH4_RS17060 Protein ID WP_014882863.1
Coordinates 3571748..3572047 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECNIH4_RS17035 (ECNIH4_17035) 3566734..3567705 - 972 WP_014882868.1 LacI family DNA-binding transcriptional regulator -
ECNIH4_RS17040 (ECNIH4_17040) 3567781..3568923 - 1143 WP_023336852.1 Gfo/Idh/MocA family oxidoreductase -
ECNIH4_RS17045 (ECNIH4_17045) 3568916..3569941 - 1026 WP_032665301.1 sugar phosphate isomerase/epimerase -
ECNIH4_RS17050 (ECNIH4_17050) 3569957..3571192 - 1236 WP_023332295.1 MFS transporter -
ECNIH4_RS17055 (ECNIH4_17055) 3571398..3571760 + 363 WP_014882864.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ECNIH4_RS17060 (ECNIH4_17060) 3571748..3572047 + 300 WP_014882863.1 helix-turn-helix transcriptional regulator Antitoxin
ECNIH4_RS17065 (ECNIH4_17065) 3572158..3573546 - 1389 WP_014882862.1 phenylalanine transporter -
ECNIH4_RS17070 (ECNIH4_17070) 3573649..3575622 - 1974 WP_023336850.1 PhoX family phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-80)


Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13952.92 Da        Isoelectric Point: 6.7114

>T49986 WP_014882864.1 NZ_CP009850:3571398-3571760 [Enterobacter cloacae]
MWNVETTDRFDQWYFEQTTALKEDVLAMMHILAEFGPTLGRPYVDTVKASAYANMKELRIQHAGNPIRAFFAFDPDRKAI
VLCAGNKTGCNQKRFYNEMITLADVEYSRHLADKEAIWQR

Download         Length: 363 bp

>T49986 NZ_CP009850:3571398-3571760 [Enterobacter cloacae]
ATGTGGAATGTCGAGACAACGGACAGATTTGATCAATGGTACTTCGAACAAACAACAGCATTAAAAGAAGATGTGCTAGC
GATGATGCATATTCTTGCTGAGTTTGGCCCAACGCTTGGGCGGCCATACGTAGATACCGTGAAGGCTTCAGCCTATGCGA
ACATGAAGGAATTACGGATTCAACATGCGGGAAATCCCATCAGAGCTTTTTTTGCTTTTGATCCTGACCGTAAGGCAATT
GTGCTCTGTGCTGGAAACAAAACCGGTTGCAATCAAAAACGGTTTTATAACGAAATGATTACGCTGGCTGACGTTGAGTA
CAGCAGACATCTGGCGGATAAGGAGGCCATATGGCAACGCTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10860.61 Da        Isoelectric Point: 9.8627

>AT49986 WP_014882863.1 NZ_CP009850:3571748-3572047 [Enterobacter cloacae]
MATLKELMAQQSAESQERIAAKVDVIRRIVALNQLREELNISQTELAAAMGVKQPTVAKIEQPGNDPRLSTLKRYVSALG
GEMSIDVTLPNGKRIAFQI

Download         Length: 300 bp

>AT49986 NZ_CP009850:3571748-3572047 [Enterobacter cloacae]
ATGGCAACGCTAAAAGAACTCATGGCACAACAGAGCGCGGAAAGTCAGGAGCGTATTGCGGCTAAAGTTGATGTAATACG
CAGAATTGTTGCCCTGAACCAACTGCGGGAAGAGCTCAATATTTCGCAGACAGAACTGGCCGCGGCAATGGGGGTAAAAC
AACCAACTGTGGCAAAAATCGAACAGCCGGGAAACGATCCCCGGCTTTCAACACTCAAACGCTATGTCAGTGCGCTCGGC
GGTGAAATGAGCATTGATGTGACGTTACCGAATGGGAAACGAATAGCGTTTCAGATTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J0PLQ3

Antitoxin

Source ID Structure
AlphaFold DB A0A2W0GCS7

References