Detailed information of TA system TA049975

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 81740..82470
• Replicon: chromosome
• Accession: NZ_CP009850
• Organism: UNVERIFIED_ORG: Enterobacter cloacae strain ECNIH4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2J0PHU0
• Locus tag: ECNIH4_RS00385
• Protein ID: WP_014881928.1
• Coordinates: 81740..82054 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ECNIH4_RS00390
• Protein ID: WP_023331687.1
• Coordinates: 82051..82470 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECNIH4_RS00360 (ECNIH4_00360)	77405..78493	+	1089	WP_014881924.1	cellulase family glycosylhydrolase	-
ECNIH4_RS00365 (ECNIH4_00365)	78396..79580	-	1185	WP_014881925.1	multidrug efflux MFS transporter EmrD	-
ECNIH4_RS00370 (ECNIH4_00370)	79756..80589	-	834	WP_014881926.1	EamA family transporter	-
ECNIH4_RS00375 (ECNIH4_00375)	80652..81098	-	447	WP_014881927.1	GNAT family N-acetyltransferase	-
ECNIH4_RS00385 (ECNIH4_00385)	81740..82054	+	315	WP_014881928.1	type II toxin-antitoxin system HigB family toxin	Toxin
ECNIH4_RS00390 (ECNIH4_00390)	82051..82470	+	420	WP_023331687.1	helix-turn-helix domain-containing protein	Antitoxin
ECNIH4_RS24815	82620..82718	+	99	WP_023331688.1	ilvB operon leader peptide IvbL	-
ECNIH4_RS00395 (ECNIH4_00395)	82825..84513	+	1689	WP_014881930.1	acetolactate synthase large subunit	-
ECNIH4_RS00400 (ECNIH4_00400)	84517..84804	+	288	WP_010426528.1	acetolactate synthase small subunit	-
ECNIH4_RS00405 (ECNIH4_00405)	84887..85480	+	594	WP_014881931.1	transcriptional regulator UhpA	-
ECNIH4_RS00410 (ECNIH4_00410)	85477..86981	+	1505	Protein_82	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-136)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12437.60 Da
• Isoelectric Point: 10.2825

>T49975 WP_014881928.1 NZ_CP009850:81740-82054 [Enterobacter cloacae]
MHLISMKAILDAVIQFPQHREELLFLGRTIEKSHCPTPVALRKIFPTLDNLKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T49975 NZ_CP009850:81740-82054 [Enterobacter cloacae]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTGATCCAGTTTCCGCAACACAGAGAGGAGCTTCTCTTTCTGGG
GCGAACCATCGAAAAGAGCCATTGCCCGACGCCTGTTGCGCTAAGAAAAATCTTTCCAACGCTAGATAATTTGAAGTATC
TCGATAAGCATTATGTTATTGATATTGCTAACAACAACCTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15561.18 Da
• Isoelectric Point: 5.6761

>AT49975 WP_023331687.1 NZ_CP009850:82051-82470 [Enterobacter cloacae]
MMIVADAMKATHALVAAVPLLGEHPNEKDYQDALELVEYLLMNEPGSPLLDIVCARIRRYEANRPEIVALRQEMESVPVG
IAVLRTLMDQYKLTISDFQDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT49975 NZ_CP009850:82051-82470 [Enterobacter cloacae]
ATGATGATCGTAGCTGATGCAATGAAAGCAACGCATGCCCTTGTCGCGGCGGTTCCTTTATTGGGTGAACATCCAAACGA
GAAAGATTACCAGGACGCACTTGAGCTGGTTGAGTATTTACTGATGAACGAACCTGGCAGCCCTTTGCTGGATATCGTAT
GTGCAAGAATTCGTCGTTACGAAGCCAATCGGCCCGAAATTGTCGCCTTACGCCAGGAGATGGAATCTGTCCCAGTGGGC
ATTGCCGTTTTAAGAACCCTTATGGATCAATACAAACTGACTATTTCGGATTTTCAGGATGAAATTGGCAGTAAATCAAT
GGTTTCAAGGGTGTTGAACGGTCAACGTCAGCTGACGTTGAACCATATAAAAAAACTGGCTGCTCGCTTTGGGGTATCGC
CTGCGCTGTTCATTGAATGA

Structures

Toxin

• AlphaFold DB: A0A2J0PHU0