Detailed information of TA system TA049931

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 1728533..1729117
• Replicon: chromosome
• Accession: NZ_CP009838
• Organism: Yersinia enterocolitica strain 2516-87

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A7U7ISC5
• Locus tag: CH48_RS08050
• Protein ID: WP_005165296.1
• Coordinates: 1728749..1729117 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: CH48_RS21405
• Protein ID: WP_129019424.1
• Coordinates: 1728533..1728685 (+)
• Length: 51 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CH48_RS08035	1723985..1725391	+	1407	WP_013650609.1	filamentous hemagglutinin N-terminal domain-containing protein	-
CH48_RS08040	1725624..1727087	-	1464	WP_005166182.1	alpha/beta hydrolase	-
CH48_RS08045	1727262..1728464	-	1203	WP_005176946.1	IS256 family transposase	-
CH48_RS21405	1728533..1728685	+	153	WP_129019424.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CH48_RS08050	1728749..1729117	+	369	WP_005165296.1	TA system toxin CbtA family protein	Toxin
CH48_RS08055	1729346..1730206	+	861	WP_013650611.1	DUF4942 domain-containing protein	-
CH48_RS08060	1730243..1731262	+	1020	WP_020282800.1	IS110 family transposase	-
CH48_RS08065	1732812..1733285	+	474	WP_032904420.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1723985..1733285	9300
	-	inside	IScluster/Tn	-	-	1727262..1731262	4000

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (1-45)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13609.55 Da
• Isoelectric Point: 6.4731

>T49931 WP_005165296.1 NZ_CP009838:1728749-1729117 [Yersinia enterocolitica]
MQTLPVTSKRAAYACLSPVIVWQSMLTYLLEQHYGLVLSDTEFSDDAVIHEHIDAGISLADALNFTVEKFDLARTDHRGF
SCQEQSPFITSIDILRARRATGLMTRMGYQTITSVIRGGKQA

Nucleotide

• Length: 369 bp

>T49931 NZ_CP009838:1728749-1729117 [Yersinia enterocolitica]
ATGCAGACCTTACCTGTCACCTCGAAACGGGCGGCCTACGCTTGCCTATCACCCGTTATTGTCTGGCAATCCATGCTCAC
GTATCTGCTAGAGCAGCATTACGGCCTGGTGCTTAGCGACACCGAGTTCAGTGATGACGCCGTTATCCACGAACATATTG
ATGCCGGTATCTCTCTGGCTGATGCGCTGAATTTCACGGTGGAGAAGTTTGACTTGGCTCGCACCGATCACCGAGGTTTC
TCCTGCCAGGAACAATCTCCCTTTATCACGTCTATCGATATTCTCCGCGCTCGTCGAGCCACCGGTCTGATGACCCGTAT
GGGTTACCAGACCATCACCTCAGTTATCCGGGGAGGAAAGCAGGCATGA

Antitoxin

Protein

• Length: 51 a.a.
• Molecular weight: 5593.44 Da
• Isoelectric Point: 4.5803

>AT49931 WP_129019424.1 NZ_CP009838:1728533-1728685 [Yersinia enterocolitica]
MELMLLSGELNPRYQHCVTLYRNGLICEADSLGSQGYVYLAIYPTPQSIA

Nucleotide

• Length: 153 bp

>AT49931 NZ_CP009838:1728533-1728685 [Yersinia enterocolitica]
CTGGAACTGATGCTGCTTTCTGGTGAGCTCAATCCCCGTTATCAGCACTGCGTCACGTTGTATCGCAATGGCTTGATCTG
CGAAGCCGATAGCCTGGGATCACAGGGTTATGTGTATCTCGCCATTTATCCTACTCCCCAATCAATTGCATAA

Structures

Toxin

• AlphaFold DB: A0A7U7ISC5