Detailed information of TA system TA049576

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-vagC/VapC-VagC
• Location: 714463..715100
• Replicon: chromosome
• Accession: NZ_CP009744
• Organism: Burkholderia contaminans strain MS14

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: NL30_RS18800
• Protein ID: WP_047851477.1
• Coordinates: 714463..714864 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A228J565
• Locus tag: NL30_RS18805
• Protein ID: WP_043184984.1
• Coordinates: 714864..715100 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NL30_RS18790	711837..712973	+	1137	WP_046547474.1	alginate lyase family protein	-
NL30_RS18795	713004..714392	+	1389	WP_047851476.1	L-serine ammonia-lyase	-
NL30_RS18800	714463..714864	-	402	WP_047851477.1	type II toxin-antitoxin system VapC family toxin	Toxin
NL30_RS18805	714864..715100	-	237	WP_043184984.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
NL30_RS18810	715463..717166	+	1704	WP_047851478.1	thiamine pyrophosphate-binding protein	-
NL30_RS18815	717163..718260	+	1098	WP_047851479.1	ADP-heptose--LPS heptosyltransferase	-
NL30_RS18820	718378..719409	-	1032	WP_047851480.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-120)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14727.75 Da
• Isoelectric Point: 4.4492

>T49576 WP_047851477.1 NZ_CP009744:c714864-714463 [Burkholderia contaminans]
MPRYMLDTNMCIFLMKSQPEQVGRRFAQCYTGDVVMSAITYAELEYGVAACASPARERGNLAALIDDIPVAPFDVAAAQA
YGPVSEATRERTKDHLDKLIAAHAVSLDVVLVTNNERDFVSYPGLRLENWLDD

Nucleotide

• Length: 402 bp

>T49576 NZ_CP009744:c714864-714463 [Burkholderia contaminans]
ATGCCGCGCTACATGCTCGACACCAACATGTGCATCTTCCTGATGAAGAGTCAGCCGGAGCAGGTTGGTAGACGGTTTGC
GCAGTGCTACACGGGGGACGTCGTGATGTCGGCGATCACGTACGCCGAGCTCGAGTACGGTGTCGCAGCGTGCGCGAGTC
CTGCGCGGGAGCGCGGCAATCTTGCCGCGCTGATCGACGATATTCCCGTCGCGCCGTTCGACGTCGCTGCCGCGCAAGCG
TATGGTCCGGTCAGCGAGGCAACCCGGGAGCGGACGAAGGATCACCTCGACAAGCTGATCGCCGCGCATGCCGTGTCGCT
CGATGTCGTACTCGTGACCAACAACGAGCGGGATTTCGTGAGCTATCCGGGACTGCGGCTGGAGAACTGGCTGGACGATT
GA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9036.18 Da
• Isoelectric Point: 6.4741

>AT49576 WP_043184984.1 NZ_CP009744:c715100-714864 [Burkholderia contaminans]
MHTTRVFRNGNSQAVRIPADLAYERSDIELEIERIGDEIRIRPARRPLTRVLEKFAKFGPDFMAEGRGDHEQAEREGL

Nucleotide

• Length: 237 bp

>AT49576 NZ_CP009744:c715100-714864 [Burkholderia contaminans]
ATGCATACGACGAGGGTATTTCGGAACGGCAACTCGCAGGCCGTGCGAATTCCTGCCGATCTTGCCTATGAACGCAGTGA
CATCGAACTGGAAATCGAGCGCATTGGTGACGAAATCCGCATTCGGCCGGCGCGGCGGCCATTGACCCGCGTCCTCGAGA
AATTCGCGAAATTCGGGCCGGATTTCATGGCGGAAGGGCGTGGTGACCACGAGCAGGCCGAACGCGAGGGTTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A228J565