Detailed information of TA system TA049243

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3146227..3146850
• Replicon: chromosome
• Accession: NZ_CP009539
• Organism: Yersinia ruckeri strain YRB

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: BD65_RS14060
• Protein ID: WP_042527784.1
• Coordinates: 3146227..3146430 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: A0A085U5C5
• Locus tag: BD65_RS14065
• Protein ID: WP_004718732.1
• Coordinates: 3146482..3146850 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BD65_RS14040	3142080..3142418	+	339	WP_007886947.1	P-II family nitrogen regulator	-
BD65_RS14045	3142457..3143746	+	1290	WP_042527778.1	ammonium transporter AmtB	-
BD65_RS14050	3143861..3144724	-	864	WP_042527780.1	acyl-CoA thioesterase II	-
BD65_RS14055	3145104..3145418	-	315	WP_042527782.1	MGMT family protein	-
BD65_RS14060	3146227..3146430	-	204	WP_042527784.1	expression modulating protein YmoA	Toxin
BD65_RS14065	3146482..3146850	-	369	WP_004718732.1	Hha toxicity modulator TomB	Antitoxin
BD65_RS16665	3148179..3148322	-	144	WP_004718738.1	type B 50S ribosomal protein L36	-
BD65_RS14075	3148342..3148596	-	255	WP_042527786.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8065.29 Da
• Isoelectric Point: 6.4573

>T49243 WP_042527784.1 NZ_CP009539:c3146430-3146227 [Yersinia ruckeri]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPATVWQHVR

Nucleotide

• Length: 204 bp

>T49243 NZ_CP009539:c3146430-3146227 [Yersinia ruckeri]
ATGACCAAAACTGACTACCTAATGCGTTTAAGAAAATGCACAACAATTGACACACTAGAGCGTGTTATTGAAAAAAATAA
GTATGAGCTATCCGATGATGAACTAGAATTATTCTATTCTGCTGCGGATCATCGTCTAGCTGAACTTACCATGAATAAGT
TATACGATAAAATTCCTGCAACTGTCTGGCAACATGTGAGGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14277.08 Da
• Isoelectric Point: 4.4225

>AT49243 WP_004718732.1 NZ_CP009539:c3146850-3146482 [Yersinia ruckeri]
MDEYSPKRHDIAQLKFLCENLYDEGIATLGDSHHGWVNDPTSAVNLQLNDLIEHIASFVMSFKIKYADEIDLCELVEEYL
DDTYTLFSSYGINDPDLRRWQKTKDRLFRLFSGEYLCALMKN

Nucleotide

• Length: 369 bp

>AT49243 NZ_CP009539:c3146850-3146482 [Yersinia ruckeri]
ATGGATGAGTACTCACCTAAGCGACATGATATTGCTCAGTTGAAATTCTTATGTGAGAATTTGTATGATGAAGGTATCGC
CACATTGGGCGATAGCCACCACGGCTGGGTTAATGATCCAACCTCCGCGGTAAATCTCCAACTGAATGATTTAATCGAAC
ACATAGCTTCATTTGTGATGAGTTTTAAAATAAAATACGCAGACGAAATCGATCTCTGCGAGTTGGTGGAGGAATATCTA
GACGATACATATACCTTGTTCAGCAGTTACGGAATCAATGATCCTGATTTACGCCGCTGGCAGAAAACAAAAGACCGATT
ATTCAGACTATTTTCAGGGGAATACCTATGTGCCCTGATGAAAAATTAA

Structures

Antitoxin

• AlphaFold DB: A0A085U5C5