Detailed information of TA system TA049240

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 1530164..1530976
• Replicon: chromosome
• Accession: NZ_CP009539
• Organism: Yersinia ruckeri strain YRB

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A0T9TM43
• Locus tag: BD65_RS06915
• Protein ID: WP_042526022.1
• Coordinates: 1530164..1530547 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: BD65_RS06920
• Protein ID: WP_042526024.1
• Coordinates: 1530608..1530976 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BD65_RS06895	1526746..1528587	+	1842	WP_004723328.1	RNA polymerase sigma factor RpoD	-
BD65_RS06905	1529003..1529851	-	849	WP_042526018.1	DUF4942 domain-containing protein	-
BD65_RS06910	1529946..1530167	-	222	WP_042526020.1	hypothetical protein	-
BD65_RS06915	1530164..1530547	-	384	WP_042526022.1	TA system toxin CbtA family protein	Toxin
BD65_RS06920	1530608..1530976	-	369	WP_042526024.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BD65_RS06925	1531068..1531388	-	321	WP_052470835.1	hypothetical protein	-
BD65_RS06930	1531399..1531878	-	480	WP_042526027.1	DNA repair protein RadC	-
BD65_RS06935	1531898..1532344	-	447	WP_042526029.1	antirestriction protein	-
BD65_RS06940	1532576..1533043	-	468	WP_172668986.1	hypothetical protein	-
BD65_RS06945	1533417..1534064	-	648	WP_042526031.1	hypothetical protein	-
BD65_RS06950	1534070..1534771	-	702	WP_042526032.1	WYL domain-containing protein	-
BD65_RS06955	1534984..1535859	-	876	WP_042526033.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14211.35 Da
• Isoelectric Point: 9.9985

>T49240 WP_042526022.1 NZ_CP009539:c1530547-1530164 [Yersinia ruckeri]
MQTVSVTPKRAAPSCPSPIAIWQVLLTHLLQQHYGLTLEDTAFSHEGVIQEHLNAGISLADALNFIVEKYELVRIDRTGF
SIREQSPFITPIDILRARQATGLMKRRGYKAITHITNGKNQRQGAHP

Nucleotide

• Length: 384 bp

>T49240 NZ_CP009539:c1530547-1530164 [Yersinia ruckeri]
ATGCAAACTGTATCGGTTACCCCGAAACGGGCGGCACCGTCTTGCCCATCACCCATCGCGATCTGGCAGGTATTGCTGAC
CCATCTATTGCAACAACACTATGGTCTGACACTGGAAGATACCGCCTTTAGTCACGAGGGTGTGATCCAAGAGCATCTTA
ATGCAGGGATTTCCCTGGCGGATGCACTGAATTTTATCGTGGAAAAATATGAACTGGTCCGTATTGATCGCACCGGTTTT
AGTATCCGTGAACAATCACCGTTTATTACGCCTATCGATATTCTCCGGGCCCGACAGGCTACTGGTCTGATGAAACGCCG
AGGTTACAAGGCCATCACTCATATCACCAACGGTAAAAACCAACGACAGGGGGCACATCCGTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13439.23 Da
• Isoelectric Point: 5.4564

>AT49240 WP_042526024.1 NZ_CP009539:c1530976-1530608 [Yersinia ruckeri]
MTDSKPDTPSQGADVTPLAWGLKRIITPQFGARLVQQGHQLHYLADRADIVGKFSDAELRHLDQVFPLWLQQLESMLMSG
ELDPRQQHCVTLHASGLTLEADTLGSCGYVYISIYPTSATTA

Nucleotide

• Length: 369 bp

>AT49240 NZ_CP009539:c1530976-1530608 [Yersinia ruckeri]
ATGACCGATAGTAAACCTGATACCCCCTCCCAGGGGGCTGATGTCACCCCACTAGCCTGGGGACTAAAACGTATCATCAC
ACCTCAGTTTGGTGCTCGCCTGGTACAGCAAGGCCACCAACTGCATTACCTGGCAGACCGTGCCGATATAGTGGGGAAGT
TCAGTGATGCCGAGTTGCGTCATCTCGACCAAGTATTCCCGCTATGGCTTCAGCAGTTGGAGTCGATGCTGATGTCGGGT
GAACTCGATCCCCGACAACAACACTGCGTCACACTCCATGCGAGCGGACTGACACTCGAAGCCGATACGTTGGGCTCCTG
CGGGTACGTGTATATATCTATCTATCCAACATCAGCTACAACTGCATAA

Structures

Toxin

• AlphaFold DB: A0A0T9TM43