Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-UPF0150
Location 3267756..3268199 Replicon chromosome
Accession NZ_CP009514
Organism Methanosarcina mazei C16

Toxin (Protein)


Gene name hicA Uniprot ID M1QEU9
Locus tag MSMAC_RS14040 Protein ID WP_011031978.1
Coordinates 3267756..3267980 (-) Length 75 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0E3RBB6
Locus tag MSMAC_RS14045 Protein ID WP_048131895.1
Coordinates 3267981..3268199 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MSMAC_RS14010 (MSMAC_2645) 3263967..3264314 + 348 WP_230625044.1 hypothetical protein -
MSMAC_RS14015 (MSMAC_2646) 3264771..3265118 + 348 WP_011031982.1 hypothetical protein -
MSMAC_RS14020 (MSMAC_2647) 3265466..3266011 + 546 WP_011031981.1 DUF6398 domain-containing protein -
MSMAC_RS19560 (MSMAC_2648) 3266416..3266565 + 150 WP_226987654.1 hypothetical protein -
MSMAC_RS14030 (MSMAC_2649) 3266872..3267231 - 360 WP_011031980.1 DUF6516 family protein -
MSMAC_RS14035 (MSMAC_2650) 3267228..3267548 - 321 WP_226987653.1 hypothetical protein -
MSMAC_RS14040 (MSMAC_2651) 3267756..3267980 - 225 WP_011031978.1 type II toxin-antitoxin system HicA family toxin Toxin
MSMAC_RS14045 (MSMAC_2652) 3267981..3268199 - 219 WP_048131895.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
MSMAC_RS14050 3268752..3268937 - 186 WP_015410807.1 hypothetical protein -
MSMAC_RS14055 (MSMAC_2653) 3269556..3270641 + 1086 WP_015410806.1 HEAT repeat domain-containing protein -
MSMAC_RS14060 (MSMAC_2654) 3270961..3272256 + 1296 WP_011031974.1 ATP-binding protein -
MSMAC_RS14070 3272801..3272998 - 198 WP_015410804.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(11-64)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-56)


Sequences

Toxin        


Download         Length: 75 a.a.        Molecular weight: 8175.81 Da        Isoelectric Point: 10.9086

>T49202 WP_011031978.1 NZ_CP009514:c3267980-3267756 [Methanosarcina mazei C16]
MSGLPVISGMQAIKAFYKAGWLPHRQVGSHVVLRKEGSKVTLTVPKHKELKPGLLRNLIKASGLTIEEFEVLLK

Download         Length: 225 bp

>T49202 NZ_CP009514:c3267980-3267756 [Methanosarcina mazei C16]
ATGTCTGGACTTCCTGTAATTTCCGGAATGCAGGCAATCAAAGCTTTTTATAAAGCTGGCTGGCTCCCTCACCGGCAGGT
AGGAAGTCATGTTGTCCTTAGAAAGGAAGGATCAAAAGTTACGCTGACTGTCCCGAAGCACAAAGAACTGAAGCCGGGCC
TTTTAAGAAATTTGATAAAAGCTTCAGGGCTGACGATAGAAGAATTCGAAGTTCTTTTAAAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 7749.76 Da        Isoelectric Point: 3.7437

>AT49202 WP_048131895.1 NZ_CP009514:c3268199-3267981 [Methanosarcina mazei C16]
MRFKIILEEDEEVGGFIASCPGLPGCFSQGDTVEEAIENIKEAIQACLESLAEDDLQECIGKSSCRVVDVVA

Download         Length: 219 bp

>AT49202 NZ_CP009514:c3268199-3267981 [Methanosarcina mazei C16]
ATGCGATTCAAAATTATCCTTGAGGAAGACGAAGAAGTCGGAGGGTTTATTGCCAGCTGTCCCGGTTTACCTGGCTGTTT
TTCACAGGGAGATACTGTAGAAGAAGCTATTGAAAATATTAAAGAAGCGATTCAGGCCTGCCTGGAATCGCTTGCAGAAG
ACGACTTGCAGGAATGCATTGGAAAATCTTCCTGTAGAGTTGTCGATGTGGTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB M1QEU9

Antitoxin

Source ID Structure
AlphaFold DB A0A0E3RBB6

References