Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-UPF0150
Location 1590351..1590794 Replicon chromosome
Accession NZ_CP009512
Organism Methanosarcina mazei S-6

Toxin (Protein)


Gene name hicA Uniprot ID M1QEU9
Locus tag MSMAS_RS06910 Protein ID WP_011031978.1
Coordinates 1590351..1590575 (-) Length 75 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0E3RBB6
Locus tag MSMAS_RS06915 Protein ID WP_048131895.1
Coordinates 1590576..1590794 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MSMAS_RS06880 (MSMAS_1296) 1586013..1586360 + 348 WP_230625044.1 hypothetical protein -
MSMAS_RS06885 (MSMAS_1297) 1587366..1587713 + 348 WP_011031982.1 hypothetical protein -
MSMAS_RS06890 (MSMAS_1298) 1588061..1588606 + 546 WP_011031981.1 DUF6398 domain-containing protein -
MSMAS_RS19435 (MSMAS_1299) 1589011..1589160 + 150 WP_226987654.1 hypothetical protein -
MSMAS_RS06900 (MSMAS_1300) 1589467..1589826 - 360 WP_011031980.1 DUF6516 family protein -
MSMAS_RS06905 (MSMAS_1301) 1589823..1590143 - 321 WP_226987653.1 hypothetical protein -
MSMAS_RS06910 (MSMAS_1302) 1590351..1590575 - 225 WP_011031978.1 type II toxin-antitoxin system HicA family toxin Toxin
MSMAS_RS06915 (MSMAS_1303) 1590576..1590794 - 219 WP_048131895.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
MSMAS_RS06920 1591347..1591532 - 186 WP_015410807.1 hypothetical protein -
MSMAS_RS06925 (MSMAS_1304) 1592151..1593236 + 1086 WP_015410806.1 HEAT repeat domain-containing protein -
MSMAS_RS06930 (MSMAS_1305) 1593556..1594851 + 1296 WP_011031974.1 ATP-binding protein -
MSMAS_RS06940 1595396..1595593 - 198 WP_015410804.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(11-64)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-56)


Sequences

Toxin        


Download         Length: 75 a.a.        Molecular weight: 8175.81 Da        Isoelectric Point: 10.9086

>T49200 WP_011031978.1 NZ_CP009512:c1590575-1590351 [Methanosarcina mazei S-6]
MSGLPVISGMQAIKAFYKAGWLPHRQVGSHVVLRKEGSKVTLTVPKHKELKPGLLRNLIKASGLTIEEFEVLLK

Download         Length: 225 bp

>T49200 NZ_CP009512:c1590575-1590351 [Methanosarcina mazei S-6]
ATGTCTGGACTTCCTGTAATTTCCGGAATGCAGGCAATCAAAGCTTTTTATAAAGCTGGCTGGCTCCCTCACCGGCAGGT
AGGAAGTCATGTTGTCCTTAGAAAGGAAGGATCAAAAGTTACGCTGACTGTCCCGAAGCACAAAGAACTGAAGCCGGGCC
TTTTAAGAAATTTGATAAAAGCTTCAGGGCTGACGATAGAAGAATTCGAAGTTCTTTTAAAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 7749.76 Da        Isoelectric Point: 3.7437

>AT49200 WP_048131895.1 NZ_CP009512:c1590794-1590576 [Methanosarcina mazei S-6]
MRFKIILEEDEEVGGFIASCPGLPGCFSQGDTVEEAIENIKEAIQACLESLAEDDLQECIGKSSCRVVDVVA

Download         Length: 219 bp

>AT49200 NZ_CP009512:c1590794-1590576 [Methanosarcina mazei S-6]
ATGCGATTCAAAATTATCCTTGAGGAAGACGAAGAAGTCGGAGGGTTTATTGCCAGCTGTCCCGGTTTACCTGGCTGTTT
TTCACAGGGAGATACTGTAGAAGAAGCTATTGAAAATATTAAAGAAGCGATTCAGGCCTGCCTGGAATCGCTTGCAGAAG
ACGACTTGCAGGAATGCATTGGAAAATCTTCCTGTAGAGTTGTCGATGTGGTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB M1QEU9

Antitoxin

Source ID Structure
AlphaFold DB A0A0E3RBB6

References