Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 719412..719977 Replicon chromosome
Accession NZ_CP009284
Organism Paenibacillus sp. FSL R7-0331

Toxin (Protein)


Gene name mazF Uniprot ID A0A089KUI4
Locus tag R70331_RS03150 Protein ID WP_042172792.1
Coordinates 719636..719977 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A089KQB8
Locus tag R70331_RS03145 Protein ID WP_042172789.1
Coordinates 719412..719642 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
R70331_RS03115 714462..715355 - 894 WP_052418895.1 ABC transporter ATP-binding protein -
R70331_RS03120 715601..716068 + 468 WP_042172782.1 polymer-forming cytoskeletal protein -
R70331_RS03125 716058..716939 - 882 WP_042172784.1 LysR family transcriptional regulator -
R70331_RS03130 717132..717698 + 567 WP_042172786.1 sugar O-acetyltransferase -
R70331_RS03135 717702..718232 + 531 WP_052418896.1 hypothetical protein -
R70331_RS03140 718262..719239 - 978 WP_042172788.1 phosphotransferase -
R70331_RS03145 719412..719642 + 231 WP_042172789.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
R70331_RS03150 719636..719977 + 342 WP_042172792.1 endoribonuclease MazF Toxin
R70331_RS03155 720092..721075 + 984 WP_042172794.1 tRNA-dihydrouridine synthase -
R70331_RS03160 721171..721740 + 570 WP_042172796.1 hypothetical protein -
R70331_RS03165 721767..722534 - 768 WP_081947448.1 response regulator transcription factor -
R70331_RS03170 722497..724668 - 2172 WP_081947818.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-110)

Antitoxin

Antitoxin PemI-like

(2-76)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12419.49 Da        Isoelectric Point: 6.9458

>T48732 WP_042172792.1 NZ_CP009284:719636-719977 [Paenibacillus sp. FSL R7-0331]
MVMDGYVPDRGDLIWLQFNPQAGHEQAGKRPALVISPASYNGKVGFSLLCPVTSKQKGYPFEVVIPNDLPIEGVILADQV
KSLDWQSRQAAFICKIPQAILSDVVAKLDLLIR

Download         Length: 342 bp

>T48732 NZ_CP009284:719636-719977 [Paenibacillus sp. FSL R7-0331]
ATGGTGATGGACGGGTACGTACCTGATCGCGGGGACTTGATATGGCTGCAGTTTAACCCGCAGGCGGGACATGAACAGGC
TGGTAAACGTCCTGCTTTAGTCATATCTCCAGCCTCTTATAATGGCAAGGTTGGCTTTTCCCTGCTCTGCCCGGTAACCT
CGAAGCAAAAGGGCTACCCGTTTGAAGTCGTTATTCCGAATGATCTGCCCATTGAAGGGGTTATCCTTGCCGATCAAGTA
AAGAGTTTAGACTGGCAGTCCCGGCAGGCAGCGTTCATCTGTAAAATACCGCAGGCAATACTATCAGATGTCGTTGCTAA
ACTGGACTTGCTGATTCGTTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8456.57 Da        Isoelectric Point: 4.5659

>AT48732 WP_042172789.1 NZ_CP009284:719412-719642 [Paenibacillus sp. FSL R7-0331]
MFQVQKWGNSLGIRIPKSLALKVGIEEGSEVDLDVEDGHLVIKPKSATLDDILSQVTTENLHKEISTGEQQGREIW

Download         Length: 231 bp

>AT48732 NZ_CP009284:719412-719642 [Paenibacillus sp. FSL R7-0331]
ATGTTCCAGGTTCAAAAATGGGGTAACAGCTTAGGCATTCGTATTCCGAAGTCCCTTGCCTTGAAGGTCGGTATTGAGGA
GGGCTCCGAAGTTGATCTCGATGTGGAGGATGGACATCTTGTAATCAAACCGAAGTCAGCTACTCTGGACGATATCCTAT
CCCAAGTCACAACGGAAAATCTCCATAAAGAGATTTCCACAGGTGAGCAGCAAGGACGGGAAATATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A089KUI4

Antitoxin

Source ID Structure
AlphaFold DB A0A089KQB8

References