Detailed information of TA system TA048728

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1509049..1509654
• Replicon: chromosome
• Accession: NZ_CP009280
• Organism: Paenibacillus sp. FSL P4-0081

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0E4H7G2
• Locus tag: P40081_RS06485
• Protein ID: WP_019913013.1
• Coordinates: 1509304..1509654 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: P40081_RS06480
• Protein ID: WP_171717509.1
• Coordinates: 1509049..1509300 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P40081_RS06460	1504601..1505164	+	564	WP_042134211.1	sigma-70 family RNA polymerase sigma factor	-
P40081_RS06465	1505161..1506060	+	900	WP_042134213.1	DUF4367 domain-containing protein	-
P40081_RS06470	1506360..1507442	+	1083	WP_042134215.1	outer membrane lipoprotein carrier protein LolA	-
P40081_RS06475	1507625..1508806	+	1182	WP_042134217.1	alanine racemase	-
P40081_RS06480	1509049..1509300	+	252	WP_171717509.1	ribbon-helix-helix protein, CopG family	Antitoxin
P40081_RS06485	1509304..1509654	+	351	WP_019913013.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
P40081_RS06490	1509953..1510498	+	546	WP_042134221.1	TetR/AcrR family transcriptional regulator	-
P40081_RS06495	1510820..1512031	+	1212	WP_042134223.1	tetracycline resistance MFS efflux pump	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12749.67 Da
• Isoelectric Point: 4.8265

>T48728 WP_019913013.1 NZ_CP009280:1509304-1509654 [Paenibacillus sp. FSL P4-0081]
MIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAAAHGFDRDSVILLEQV
RTIDKQRLTDKITHLDDETMKLVDDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T48728 NZ_CP009280:1509304-1509654 [Paenibacillus sp. FSL P4-0081]
TTGATTGTTAAACGCGGCGACGTTTTTTTTGCCGACCTTTCACCGGTCGTGGGTTCAGAGCAAGGCGGAGTTAGGCCGGT
ACTGGTCATTCAGAACGACATTGGCAATCGCTTCAGCCCGACTGTAATCGTGGCAGCTATCACTGCCCAGATCCAGAAGG
CCAAACTGCCGACGCATGTGGAGATTGATGCGGCTGCCCATGGCTTTGACCGGGATTCCGTTATATTGCTGGAGCAGGTT
CGGACCATTGACAAACAACGCTTAACCGATAAGATCACCCACCTGGACGATGAGACCATGAAGCTGGTGGATGATTCGCT
GCAAATCAGCCTGGGTCTGATTGACTTCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9499.94 Da
• Isoelectric Point: 5.1186

>AT48728 WP_171717509.1 NZ_CP009280:1509049-1509300 [Paenibacillus sp. FSL P4-0081]
MISLPDHLLQEVDGIVQLENSNRSELIRQAMKLYLTERRKRTIRESMQRGYMEMAKINLTMASEAFLAEEDADSTLGRLV
SGV

Nucleotide

• Length: 252 bp

>AT48728 NZ_CP009280:1509049-1509300 [Paenibacillus sp. FSL P4-0081]
ATGATCAGCTTGCCCGATCATCTCTTGCAGGAAGTGGATGGGATCGTTCAATTGGAGAACTCTAACCGTAGTGAATTGAT
CAGGCAGGCCATGAAGCTGTACTTGACCGAACGGAGGAAACGTACCATCCGGGAGTCGATGCAGCGGGGATACATGGAAA
TGGCTAAGATTAATTTGACCATGGCAAGCGAGGCCTTTCTCGCAGAGGAAGATGCAGACAGTACTCTTGGCCGCTTAGTA
AGCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: A0A0E4H7G2