Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5423852..5424477 Replicon chromosome
Accession NZ_CP009274
Organism Klebsiella variicola strain DX120E

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag KR75_RS25515 Protein ID WP_008807903.1
Coordinates 5423852..5424235 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KR75_RS25520 Protein ID WP_004150355.1
Coordinates 5424235..5424477 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KR75_RS25500 5421219..5422121 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KR75_RS25505 5422118..5422753 + 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
KR75_RS25510 5422750..5423679 + 930 WP_039102683.1 formate dehydrogenase accessory protein FdhE -
KR75_RS25515 5423852..5424235 - 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
KR75_RS25520 5424235..5424477 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
KR75_RS25525 5424682..5425599 + 918 WP_039102682.1 alpha/beta hydrolase -
KR75_RS25530 5425614..5426555 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
KR75_RS25535 5426600..5427037 - 438 WP_039102681.1 D-tyrosyl-tRNA(Tyr) deacylase -
KR75_RS25540 5427034..5427894 - 861 WP_008807907.1 virulence factor BrkB family protein -
KR75_RS25545 5427888..5428487 - 600 WP_008807908.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T48724 WP_008807903.1 NZ_CP009274:c5424235-5423852 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

>T48724 NZ_CP009274:c5424235-5423852 [Klebsiella variicola]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTAAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCACTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTGATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAACTACACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT48724 WP_004150355.1 NZ_CP009274:c5424477-5424235 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT48724 NZ_CP009274:c5424477-5424235 [Klebsiella variicola]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References