Detailed information of TA system TA048487

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 524569..526127
• Replicon: chromosome
• Accession: NZ_CP009159
• Organism: Actinobacillus suis ATCC 33415

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: K0FW50
• Locus tag: ASU1_RS02410
• Protein ID: WP_014991250.1
• Coordinates: 524805..526127 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: K0G332
• Locus tag: ASU1_RS02405
• Protein ID: WP_014991249.1
• Coordinates: 524569..524811 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASU1_RS02400	520837..524445	+	3609	WP_014991248.1	exodeoxyribonuclease V subunit beta	-
ASU1_RS02405	524569..524811	+	243	WP_014991249.1	helix-turn-helix domain-containing protein	Antitoxin
ASU1_RS02410	524805..526127	+	1323	WP_014991250.1	HipA domain-containing protein	Toxin
ASU1_RS02415	526191..527441	-	1251	WP_014991251.1	NupC/NupG family nucleoside CNT transporter	-
ASU1_RS02420	527481..528269	-	789	WP_014991252.1	4Fe-4S cluster-binding domain-containing protein	-
ASU1_RS02425	528271..529815	-	1545	WP_014991253.1	YjjI family glycine radical enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (7-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-64)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 50395.13 Da
• Isoelectric Point: 8.3234

>T48487 WP_014991250.1 NZ_CP009159:524805-526127 [Actinobacillus suis ATCC 33415]
MVKCLCVLMNGELVGFFERTMSGSHLFRYADEWFLSPNVRPISLSMPLRREVYQGKEVINFFDNLLPDHPQVRARIAARF
QAETQQPFELLAKIGRDATGAITLLEENTEIPNHKVIYATELNENELHSLLLGYQQNAPLGMLDEAQDFRISIAGAQEKT
ALLWYNERWQLPHGLTPTTHIIKLPIGKIQTPKYQLDLSQSVDNELICLRLCAAFGLPTANVNLLEVGDVRALVVERFDR
KLHSSNKYFLRLPYEDFCQVFGLAPTQKYESDGGIGIRHIMDKLLYSSHTSDRAQFMKTQFLFWVLGAIDGHAKNFSIAL
KSQSRYHLSPIYDVISAYPMLGGKGWHQSDLQLAMSLYSTQKGKKWSWQKIRPVHYLSTAKSVGFNQLEMKHIIEEISDT
ALKKLDFVADRLPININEQVRDQIFNGVKKQIDKIKLDRI

Nucleotide

• Length: 1323 bp

>T48487 NZ_CP009159:524805-526127 [Actinobacillus suis ATCC 33415]
ATGGTGAAGTGTTTATGTGTGTTAATGAATGGGGAATTAGTTGGTTTTTTTGAGCGTACTATGTCCGGTTCGCATTTATT
CCGTTATGCAGATGAGTGGTTTTTATCTCCAAATGTTAGACCTATTTCTCTTTCTATGCCATTAAGAAGGGAGGTTTATC
AAGGTAAAGAAGTAATTAATTTTTTTGATAACTTATTACCTGATCACCCTCAAGTTAGAGCTAGAATTGCTGCGCGATTT
CAAGCTGAAACACAACAACCTTTTGAATTGTTAGCTAAGATTGGACGAGATGCAACCGGAGCAATAACCCTATTAGAAGA
AAACACAGAAATTCCCAATCATAAAGTTATTTATGCAACGGAATTAAACGAAAATGAACTTCATTCGTTATTATTAGGCT
ATCAGCAAAATGCACCACTTGGCATGTTGGATGAAGCACAAGATTTTCGAATTTCTATCGCAGGGGCTCAAGAAAAGACA
GCATTATTATGGTATAACGAACGTTGGCAATTACCCCATGGATTAACCCCAACGACACATATTATTAAATTACCGATAGG
TAAAATACAAACACCTAAATATCAATTAGATCTGTCTCAAAGTGTAGACAACGAATTAATTTGTTTACGCTTATGTGCTG
CCTTCGGTTTACCGACTGCAAATGTAAATTTATTAGAGGTCGGGGATGTGCGAGCATTGGTTGTAGAACGGTTTGATCGT
AAATTACATTCTTCAAATAAATATTTTTTACGCTTACCTTATGAAGATTTTTGCCAAGTATTCGGCTTAGCGCCAACCCA
AAAATATGAGAGTGATGGTGGTATCGGTATTCGACACATTATGGATAAACTTTTGTATTCCAGTCATACTTCGGATCGTG
CACAATTCATGAAAACCCAATTTTTATTTTGGGTGCTTGGAGCAATAGATGGGCATGCCAAAAACTTTTCTATTGCATTG
AAGTCGCAGAGTCGTTATCACTTATCGCCAATTTATGATGTTATATCGGCTTATCCAATGCTGGGAGGTAAAGGATGGCA
TCAGAGTGATTTGCAACTTGCGATGAGTCTGTATAGTACCCAAAAAGGTAAGAAATGGAGTTGGCAAAAAATACGACCGG
TACATTATCTGTCTACCGCTAAATCTGTCGGATTTAATCAGTTAGAAATGAAACATATTATTGAAGAGATTTCAGATACG
GCATTAAAAAAGTTAGATTTTGTAGCTGATAGGCTGCCAATAAATATTAATGAACAAGTAAGAGATCAAATTTTTAATGG
AGTCAAAAAACAGATTGATAAAATCAAATTAGATAGGATTTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9379.79 Da
• Isoelectric Point: 8.6782

>AT48487 WP_014991249.1 NZ_CP009159:524569-524811 [Actinobacillus suis ATCC 33415]
MLINDPNSLAVYLRDIRKQQKQSQKMIAQRVGLRQATISTFEAQPDKVTIETLFKIVHSLGLEIHLEEKHTNMFNRSDEW

Nucleotide

• Length: 243 bp

>AT48487 NZ_CP009159:524569-524811 [Actinobacillus suis ATCC 33415]
ATGCTAATCAATGATCCTAATAGTTTAGCCGTTTACCTACGTGATATTCGTAAGCAGCAGAAACAATCACAAAAAATGAT
TGCTCAACGAGTCGGTTTGCGACAAGCAACGATTTCTACATTCGAGGCCCAGCCTGATAAAGTCACAATAGAAACGCTAT
TTAAAATAGTTCATTCTCTTGGTTTAGAAATTCATTTAGAAGAAAAGCATACGAATATGTTTAATAGGAGCGATGAATGG
TGA

Structures

Toxin

• AlphaFold DB: K0FW50

Antitoxin

• AlphaFold DB: K0G332