Detailed information of TA system TA048486

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-YefM
• Location: 90033..90742
• Replicon: chromosome
• Accession: NZ_CP009159
• Organism: Actinobacillus suis ATCC 33415

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: K0G9D3
• Locus tag: ASU1_RS00500
• Protein ID: WP_014990917.1
• Coordinates: 90320..90742 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: K0G3K1
• Locus tag: ASU1_RS00495
• Protein ID: WP_014990916.1
• Coordinates: 90033..90320 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASU1_RS00480	85432..86613	+	1182	WP_014990913.1	bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN	-
ASU1_RS00485	86668..87216	+	549	WP_014990914.1	type IV pilus biogenesis/stability protein PilW	-
ASU1_RS00490	87292..89631	+	2340	WP_014990915.1	RNA-binding transcriptional accessory protein	-
ASU1_RS00495	90033..90320	+	288	WP_014990916.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ASU1_RS00500	90320..90742	+	423	WP_014990917.1	type II toxin-antitoxin system VapC family toxin	Toxin
ASU1_RS00505	91117..92397	-	1281	WP_014990918.1	NAD(P)/FAD-dependent oxidoreductase	-
ASU1_RS00510	92509..93027	-	519	WP_179130574.1	SprT family zinc-dependent metalloprotease	-
ASU1_RS00515	93028..93798	-	771	WP_014990920.1	ribonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-53)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 16233.07 Da
• Isoelectric Point: 9.0431

>T48486 WP_014990917.1 NZ_CP009159:90320-90742 [Actinobacillus suis ATCC 33415]
MYLLDTNIVSELRKMESGKADVNVVAWFQQVNLTDAYLSVITLFEIKLGILQLKRKDIQQAKLLQSWFEQKLLHHFENRI
LPLDTKVMMICAEFHIPDKKPLNDSYIAATAKAHRFKIVTRNVKNFKGCGVEVLNPFIDE

Nucleotide

• Length: 423 bp

>T48486 NZ_CP009159:90320-90742 [Actinobacillus suis ATCC 33415]
ATGTATTTATTAGATACCAATATTGTTTCCGAACTACGTAAAATGGAAAGTGGAAAAGCTGATGTTAATGTGGTTGCCTG
GTTTCAACAAGTCAATTTAACCGATGCTTATTTAAGTGTTATCACTCTCTTTGAAATTAAGTTAGGTATTTTGCAACTTA
AACGCAAAGATATTCAACAAGCTAAACTTTTACAAAGTTGGTTTGAACAAAAACTTTTACATCATTTTGAAAATAGAATT
TTACCGCTTGATACCAAAGTTATGATGATCTGTGCAGAGTTTCATATTCCGGATAAAAAGCCGTTAAATGACTCTTATAT
AGCCGCTACGGCTAAAGCACATAGATTTAAAATTGTGACGAGAAATGTGAAAAATTTTAAAGGATGTGGGGTAGAGGTAC
TGAATCCATTTATTGATGAATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10623.95 Da
• Isoelectric Point: 5.0203

>AT48486 WP_014990916.1 NZ_CP009159:90033-90320 [Actinobacillus suis ATCC 33415]
MAVISSKEFNQRASAVLKIAETEPVYITKWGKVVSVLASYAHYQAQTKSQSLADAFSSTVPTAILSTEAEQQFEQTLQQI
RQQSKMNVPDLSEWE

Nucleotide

• Length: 288 bp

>AT48486 NZ_CP009159:90033-90320 [Actinobacillus suis ATCC 33415]
ATGGCGGTCATCAGTTCCAAAGAATTTAACCAACGTGCCAGTGCTGTATTAAAAATTGCAGAAACCGAGCCTGTGTATAT
TACTAAATGGGGTAAGGTGGTTAGTGTGTTGGCAAGCTACGCTCATTATCAAGCTCAAACGAAATCGCAAAGTTTGGCAG
ATGCTTTTAGCAGTACGGTACCAACTGCAATATTGAGTACCGAAGCCGAACAGCAATTTGAGCAAACTTTGCAGCAAATA
CGTCAACAAAGCAAAATGAACGTACCGGATTTATCGGAGTGGGAATAA

Structures

Toxin

• AlphaFold DB: K0G9D3

Antitoxin

• AlphaFold DB: K0G3K1