Detailed information of TA system TA048454

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 210181..210779
• Replicon: plasmid pSymA
• Accession: NZ_CP009145
• Organism: Sinorhizobium meliloti strain RMO17

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q930F0
• Locus tag: DU99_RS18365
• Protein ID: WP_010967243.1
• Coordinates: 210486..210779 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q930F1
• Locus tag: DU99_RS18360
• Protein ID: WP_010967242.1
• Coordinates: 210181..210489 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DU99_RS18345	206382..208166	+	1785	WP_040120576.1	polysaccharide biosynthesis/export family protein	-
DU99_RS18350	209206..209472	+	267	Protein_200	EAL domain-containing protein	-
DU99_RS18355	209624..209836	-	213	WP_040120577.1	hypothetical protein	-
DU99_RS18360	210181..210489	+	309	WP_010967242.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
DU99_RS18365	210486..210779	+	294	WP_010967243.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
DU99_RS18370	211012..212211	-	1200	WP_040120891.1	NAD-dependent formate dehydrogenase	-
DU99_RS18375	212415..212741	+	327	WP_080726196.1	hypothetical protein	-
DU99_RS18380	213159..214058	-	900	WP_010967198.1	LysR family transcriptional regulator	-
DU99_RS18385	214170..215102	+	933	WP_040120578.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1466845	1466845

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-53)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11278.93 Da
• Isoelectric Point: 6.4734

>T48454 WP_010967243.1 NZ_CP009145:210486-210779 [Sinorhizobium meliloti]
MRLVWARYALDDRDTIFSYIERENPRAAVHVDEEIVSAVRRLLDFPESGRPGRIAGTRELVIPRTPYIAAYMVMEDRIRI
LRVLHGAQKWPSELDDG

Nucleotide

• Length: 294 bp

>T48454 NZ_CP009145:210486-210779 [Sinorhizobium meliloti]
ATGAGGCTCGTCTGGGCGCGATATGCGCTTGACGATCGCGACACCATCTTCAGTTACATCGAAAGGGAGAATCCGAGGGC
GGCGGTCCACGTCGACGAGGAGATCGTCAGCGCGGTACGTCGTCTGCTTGATTTTCCGGAAAGCGGCCGCCCTGGCCGGA
TTGCCGGAACTCGAGAGCTTGTTATCCCTCGGACCCCCTACATTGCAGCCTACATGGTAATGGAGGATAGAATTCGAATT
CTGCGTGTCCTGCACGGCGCCCAGAAGTGGCCCAGCGAGCTCGACGACGGGTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11210.58 Da
• Isoelectric Point: 5.6604

>AT48454 WP_010967242.1 NZ_CP009145:210181-210489 [Sinorhizobium meliloti]
MASNALVQTRIDAEVKERATAVLENMGLTVSDAVRILLTRTANEGALPLELFSHSEAHDAWFRAKVLRALEDTRPDVDDA
DADAHFRERRAAALRKAAAGDR

Nucleotide

• Length: 309 bp

>AT48454 NZ_CP009145:210181-210489 [Sinorhizobium meliloti]
ATGGCTTCAAATGCACTTGTTCAGACTCGAATCGATGCCGAGGTCAAAGAGCGCGCCACGGCGGTTTTAGAGAACATGGG
TCTGACGGTCTCGGACGCTGTTCGAATCCTGTTAACTCGGACGGCGAATGAGGGCGCCCTGCCGCTGGAACTCTTCAGCC
ACAGCGAGGCGCACGACGCTTGGTTCCGCGCCAAAGTGTTGCGGGCACTCGAGGATACCCGACCCGACGTCGACGATGCC
GACGCCGATGCGCACTTTCGAGAGCGCCGGGCGGCAGCTTTGCGCAAGGCTGCGGCGGGTGATCGATGA

Structures

Toxin

• AlphaFold DB: Q930F0

Antitoxin

• AlphaFold DB: Q930F1