Detailed information of TA system TA048247

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 753007..753640
• Replicon: chromosome
• Accession: NZ_CP009100
• Organism: Mycobacterium tuberculosis strain ZMC13-264

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IZ84_RS03435
• Protein ID: WP_003912919.1
• Coordinates: 753007..753390 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ56
• Locus tag: IZ84_RS03440
• Protein ID: WP_003403368.1
• Coordinates: 753485..753640 (-)
• Length: 52 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IZ84_RS03410	748299..748835	+	537	WP_003403341.1	50S ribosomal protein L10	-
IZ84_RS03415	748872..749264	+	393	WP_003403353.1	50S ribosomal protein L7/L12	-
IZ84_RS03420	749257..749952	-	696	WP_003403355.1	TetR/AcrR family transcriptional regulator	-
IZ84_RS03425	750023..751528	+	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
IZ84_RS03430	751609..752619	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
IZ84_RS03435	753007..753390	-	384	WP_003912919.1	ribonuclease VapC6	Toxin
IZ84_RS03440	753485..753640	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
IZ84_RS03445	753716..754432	-	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
IZ84_RS03450	754708..755016	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
IZ84_RS03455	755003..755248	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	-
IZ84_RS03460	755357..755794	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	-
IZ84_RS03465	755791..756045	-	255	WP_003911263.1	antitoxin VapB7	-
IZ84_RS03470	756159..758522	+	2364	WP_003901895.1	arylsulfatase AtsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-44)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13989.76 Da
• Isoelectric Point: 6.0803

>T48247 WP_003912919.1 NZ_CP009100:c753390-753007 [Mycobacterium tuberculosis]
MAAATTTGTHRGLELRAAQRAVGSCEPQRAEFCRSARNADEFDQMSRMFGDVYPDVPVPKSVWRWIDSAQHRLARAGAVG
ALSVVDLLICDTATARGLVVLHDDADYELAERHLPDIRVRRVVSADD

Nucleotide

• Length: 384 bp

>T48247 NZ_CP009100:c753390-753007 [Mycobacterium tuberculosis]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTGGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGATCTGCGACACTGCGACGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin

Protein

• Length: 52 a.a.
• Molecular weight: 5872.72 Da
• Isoelectric Point: 5.7734

>AT48247 WP_003403368.1 NZ_CP009100:c753640-753485 [Mycobacterium tuberculosis]
VSVTQIDLDDEALADVMRIAAVHTKKEAVNLAMRDYVERFRRIEALARSRE

Nucleotide

• Length: 156 bp

>AT48247 NZ_CP009100:c753640-753485 [Mycobacterium tuberculosis]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGATGTGATGCGCATCGCCGCCGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U4BSF8