Detailed information of TA system TA048235

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 71652..72285
• Replicon: chromosome
• Accession: NZ_CP009100
• Organism: Mycobacterium tuberculosis strain ZMC13-264

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IZ84_RS00355
• Protein ID: WP_003902592.1
• Coordinates: 71884..72285 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P0CW29
• Locus tag: IZ84_RS00350
• Protein ID: WP_003400577.1
• Coordinates: 71652..71891 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IZ84_RS00340	66948..68408	+	1461	WP_038435763.1	FAD-binding oxidoreductase	-
IZ84_RS20735	68444..68605	+	162	WP_003899796.1	hypothetical protein	-
IZ84_RS00345	68646..71585	+	2940	WP_003907192.1	UPF0182 family protein	-
IZ84_RS00350	71652..71891	+	240	WP_003400577.1	antitoxin VapB1	Antitoxin
IZ84_RS00355	71884..72285	+	402	WP_003902592.1	type II toxin-antitoxin system ribonuclease VapC1	Toxin
IZ84_RS00360	72337..74574	-	2238	WP_003899797.1	NADP-dependent isocitrate dehydrogenase	-
IZ84_RS00365	74692..75261	-	570	WP_003400591.1	TetR/AcrR family transcriptional regulator; helix-turn-helix transcriptional regulator	-
IZ84_RS00370	75364..76275	+	912	WP_003899798.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (24-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14349.48 Da
• Isoelectric Point: 4.8887

>T48235 WP_003902592.1 NZ_CP009100:71884-72285 [Mycobacterium tuberculosis]
VDECVVDAAAVVDALAGKGASAIVLRGLLKEPISNAPHLLDAEVGHALRRAVLSDEISEEQARAALDALPYLIDNRYPHS
PRLIEYTWQLRHNVTFYDALYVALATALDVPLLTGDSRLAAAPGLPCEIKLVR

Nucleotide

• Length: 402 bp

>T48235 NZ_CP009100:71884-72285 [Mycobacterium tuberculosis]
GTGGATGAATGTGTAGTCGACGCGGCGGCCGTGGTTGACGCTCTCGCCGGCAAGGGCGCCAGCGCGATCGTTCTGCGCGG
TTTGCTCAAGGAGCCGATTTCTAACGCGCCGCATTTGCTGGACGCAGAGGTCGGACATGCACTCCGCCGCGCCGTGCTCA
GCGACGAAATCTCCGAAGAGCAGGCTCGCGCCGCGTTGGATGCCTTGCCTTATCTCATCGACAATCGTTACCCGCACAGC
CCACGACTGATCGAATACACATGGCAGCTAAGGCACAACGTCACGTTCTACGACGCCCTTTACGTCGCACTGGCCACCGC
ACTGGATGTCCCGCTGCTCACGGGCGACTCGCGGCTTGCGGCCGCGCCGGGCCTTCCGTGCGAAATCAAACTCGTTCGGT
GA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8717.84 Da
• Isoelectric Point: 8.5960

>AT48235 WP_003400577.1 NZ_CP009100:71652-71891 [Mycobacterium tuberculosis]
MATIQVRDLPEDVAETYRRRATAAGQSLQTYMRTKLIEGVRGRDKAEAIEILEQALASTASPGISRETIEASRRELRGG

Nucleotide

• Length: 240 bp

>AT48235 NZ_CP009100:71652-71891 [Mycobacterium tuberculosis]
ATGGCTACCATTCAAGTTCGGGATTTGCCCGAAGATGTCGCCGAAACCTATCGACGGCGCGCCACCGCAGCGGGGCAGTC
GCTGCAGACGTATATGCGCACCAAGCTCATCGAAGGGGTGCGGGGCCGAGACAAGGCCGAGGCAATCGAGATCCTGGAAC
AGGCGCTCGCCAGCACTGCCAGCCCAGGCATCAGCCGGGAGACCATCGAGGCATCCCGGCGGGAGCTCAGGGGTGGATGA

Structures

Antitoxin

• AlphaFold DB: A0A829CHM7