Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 5039251..5039753 Replicon chromosome
Accession NZ_CP009072
Organism Escherichia coli ATCC 25922

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag DR76_RS25010 Protein ID WP_000767822.1
Coordinates 5039251..5039505 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag DR76_RS25015 Protein ID WP_001259255.1
Coordinates 5039502..5039753 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DR76_RS24995 5035744..5037102 - 1359 WP_000019194.1 putrescine/proton symporter PlaP -
DR76_RS29405 5037092..5037154 - 63 WP_010723108.1 membrane protein YoeI -
DR76_RS25000 5037369..5038298 - 930 WP_000803366.1 LysR family transcriptional regulator -
DR76_RS25005 5038344..5039168 - 825 WP_000754733.1 SDR family oxidoreductase -
DR76_RS25010 5039251..5039505 - 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
DR76_RS25015 5039502..5039753 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
DR76_RS28060 5040036..5040086 + 51 WP_001364200.1 his operon leader peptide -
DR76_RS25020 5040232..5041131 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
DR76_RS25025 5041137..5042441 + 1305 WP_001297912.1 histidinol dehydrogenase -
DR76_RS25030 5042438..5043508 + 1071 WP_000108995.1 histidinol-phosphate transaminase -
DR76_RS25035 5043508..5044575 + 1068 WP_000080122.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T48232 WP_000767822.1 NZ_CP009072:c5039505-5039251 [Escherichia coli ATCC 25922]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T48232 NZ_CP009072:c5039505-5039251 [Escherichia coli ATCC 25922]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT48232 WP_001259255.1 NZ_CP009072:c5039753-5039502 [Escherichia coli ATCC 25922]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT48232 NZ_CP009072:c5039753-5039502 [Escherichia coli ATCC 25922]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References