Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 501052..501670 Replicon chromosome
Accession NZ_CP009050
Organism Escherichia coli NCCP15648

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag A610_RS02465 Protein ID WP_001291435.1
Coordinates 501052..501270 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag A610_RS02470 Protein ID WP_000344800.1
Coordinates 501296..501670 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A610_RS02430 496342..496914 + 573 WP_000779826.1 lipoprotein -
A610_RS02435 496945..497256 - 312 WP_000409911.1 MGMT family protein -
A610_RS02445 497635..497988 + 354 WP_000878140.1 DUF1428 domain-containing protein -
A610_RS02450 498030..499580 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
A610_RS02455 499744..500214 - 471 WP_000136192.1 YlaC family protein -
A610_RS02460 500330..500881 - 552 WP_000102564.1 maltose O-acetyltransferase -
A610_RS02465 501052..501270 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
A610_RS02470 501296..501670 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
A610_RS02475 502216..505365 - 3150 Protein_456 efflux RND transporter permease AcrB -
A610_RS02480 505388..506581 - 1194 WP_001386618.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T48175 WP_001291435.1 NZ_CP009050:c501270-501052 [Escherichia coli NCCP15648]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T48175 NZ_CP009050:c501270-501052 [Escherichia coli NCCP15648]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT48175 WP_000344800.1 NZ_CP009050:c501670-501296 [Escherichia coli NCCP15648]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT48175 NZ_CP009050:c501670-501296 [Escherichia coli NCCP15648]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References