Detailed information of TA system TA047827

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5697110..5697705
• Replicon: chromosome
• Accession: NZ_CP008871
• Organism: Pseudomonas aeruginosa strain W45909

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: HV96_RS27315
• Protein ID: WP_003117425.1
• Coordinates: 5697427..5697705 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV96_RS27310
• Protein ID: WP_003113527.1
• Coordinates: 5697110..5697415 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV96_RS27275	5692249..5693097	+	849	WP_023114865.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
HV96_RS27285	5693264..5694205	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
HV96_RS27290	5694322..5694936	+	615	WP_003095013.1	50S ribosomal protein L25/general stress protein Ctc	-
HV96_RS27295	5694978..5695562	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
HV96_RS27300	5695603..5696703	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
HV96_RS27310	5697110..5697415	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
HV96_RS27315	5697427..5697705	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV96_RS27325	5698034..5700262	+	2229	WP_003113525.1	TonB-dependent receptor	-
HV96_RS27330	5700332..5700979	-	648	WP_003095021.1	carbonate dehydratase	-
HV96_RS27335	5701041..5702279	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T47827 WP_003117425.1 NZ_CP008871:c5697705-5697427 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T47827 NZ_CP008871:c5697705-5697427 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT47827 WP_003113527.1 NZ_CP008871:c5697415-5697110 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT47827 NZ_CP008871:c5697415-5697110 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A241XLJ5