Detailed information of TA system TA047823

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2708280..2709322
• Replicon: chromosome
• Accession: NZ_CP008871
• Organism: Pseudomonas aeruginosa strain W45909

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: HV96_RS13215
• Protein ID: WP_003153636.1
• Coordinates: 2708747..2709322 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: HV96_RS13210
• Protein ID: WP_003050245.1
• Coordinates: 2708280..2708750 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV96_RS13175	2703672..2705090	-	1419	WP_006226029.1	TIGR03752 family integrating conjugative element protein	-
HV96_RS13180	2705080..2705991	-	912	WP_006226028.1	TIGR03749 family integrating conjugative element protein	-
HV96_RS13185	2705988..2706680	-	693	WP_003090182.1	TIGR03746 family integrating conjugative element protein	-
HV96_RS13190	2706677..2707075	-	399	WP_003050133.1	TIGR03750 family conjugal transfer protein	-
HV96_RS13195	2707087..2707446	-	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
HV96_RS13200	2707463..2707696	-	234	WP_003090170.1	TIGR03758 family integrating conjugative element protein	-
HV96_RS13205	2707693..2708076	-	384	WP_003090167.1	RAQPRD family integrative conjugative element protein	-
HV96_RS13210	2708280..2708750	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
HV96_RS13215	2708747..2709322	+	576	WP_003153636.1	PIN domain-containing protein	Toxin
HV96_RS13220	2709340..2710254	+	915	WP_023114497.1	AAA family ATPase	-
HV96_RS13225	2710251..2710721	+	471	WP_023114496.1	hypothetical protein	-
HV96_RS13230	2710718..2711218	+	501	WP_003090159.1	hypothetical protein	-
HV96_RS13235	2711218..2712120	+	903	WP_003090158.1	nucleotidyltransferase domain-containing protein	-
HV96_RS13240	2712159..2712884	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2633716..2755438	121722

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T47823 WP_003153636.1 NZ_CP008871:2708747-2709322 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T47823 NZ_CP008871:2708747-2709322 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAATGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT47823 WP_003050245.1 NZ_CP008871:2708280-2708750 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT47823 NZ_CP008871:2708280-2708750 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68