Detailed information of TA system TA047640

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 4813349..4813965
• Replicon: chromosome
• Accession: NZ_CP008823
• Organism: UNVERIFIED_ORG: Enterobacter cloacae ECNIH2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: ECNIH2_RS23095
• Protein ID: WP_015569913.1
• Coordinates: 4813349..4813720 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: ECNIH2_RS23100
• Protein ID: WP_015569912.1
• Coordinates: 4813723..4813965 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECNIH2_RS23080 (ECNIH2_23195)	4810849..4811751	+	903	WP_014072386.1	formate dehydrogenase subunit beta	-
ECNIH2_RS23085 (ECNIH2_23200)	4811748..4812383	+	636	WP_015569914.1	formate dehydrogenase cytochrome b556 subunit	-
ECNIH2_RS23090 (ECNIH2_23205)	4812380..4813309	+	930	WP_003861956.1	formate dehydrogenase accessory protein FdhE	-
ECNIH2_RS23095 (ECNIH2_23210)	4813349..4813720	-	372	WP_015569913.1	PIN domain-containing protein	Toxin
ECNIH2_RS23100 (ECNIH2_23215)	4813723..4813965	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
ECNIH2_RS23105 (ECNIH2_23220)	4814164..4815084	+	921	WP_015569911.1	alpha/beta hydrolase	-
ECNIH2_RS23110 (ECNIH2_23225)	4815093..4816034	-	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
ECNIH2_RS23115 (ECNIH2_23230)	4816079..4816516	-	438	WP_015569909.1	D-aminoacyl-tRNA deacylase	-
ECNIH2_RS23120 (ECNIH2_23235)	4816513..4817394	-	882	WP_003861949.1	virulence factor BrkB family protein	-
ECNIH2_RS23125 (ECNIH2_23240)	4817388..4817987	-	600	WP_003861947.1	glucose-1-phosphatase	-
ECNIH2_RS23130 (ECNIH2_23245)	4818106..4818906	-	801	WP_003861944.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13767.90 Da
• Isoelectric Point: 6.4882

>T47640 WP_015569913.1 NZ_CP008823:c4813720-4813349 [Enterobacter cloacae ECNIH2]
MEHMAVFDTNILIDLFNNRIEAADAIEHTASHRAISLITWMEVMVSARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T47640 NZ_CP008823:c4813720-4813349 [Enterobacter cloacae ECNIH2]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTATTGAAGCTGCGGATGCGATTGA
GCATACCGCGTCGCATCGTGCCATCAGCTTAATTACCTGGATGGAAGTAATGGTGAGCGCTCGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTTATGGGCGCTTTTGAAATTATTGATGTGTCTCGGGATATTGCGGAAAGAAGCGTTTTACTACGT
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCGCAGAGCAGAAAATGTCCGTTGATTTCTCGCAA
CACGAAAGATTTTGCGGGTATTGACGAGGTCCTTACGCCTTACCAGGTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT47640 WP_015569912.1 NZ_CP008823:c4813965-4813723 [Enterobacter cloacae ECNIH2]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT47640 NZ_CP008823:c4813965-4813723 [Enterobacter cloacae ECNIH2]
ATGAGCGTTTTGGCGGAACTTGATATGGGCAGAATACTTATCGATTTGTCTGATGACGTGATTCAACGTTTGGATAATCT
CAAGCAATTGCGAAATCAACCACGTGCTGAACTGCTTCGGGAAGCGATAGAGCTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3