Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 3963627..3964217 Replicon chromosome
Accession NZ_CP008788
Organism Klebsiella oxytoca KONIH1

Toxin (Protein)


Gene name pemK Uniprot ID A0A0H3HDW1
Locus tag KONIH1_RS18510 Protein ID WP_014229979.1
Coordinates 3963627..3963959 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID J6I3K7
Locus tag KONIH1_RS18515 Protein ID WP_004852307.1
Coordinates 3963960..3964217 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KONIH1_RS18485 3959040..3959297 + 258 WP_014229908.1 histidine kinase -
KONIH1_RS18490 3959430..3960884 + 1455 WP_014229909.1 AMP nucleosidase -
KONIH1_RS18500 3961847..3963304 - 1458 WP_014229916.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
KONIH1_RS18510 3963627..3963959 - 333 WP_014229979.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KONIH1_RS18515 3963960..3964217 - 258 WP_004852307.1 antitoxin Antitoxin
KONIH1_RS34630 3964928..3965008 + 81 Protein_3683 molybdopterin-binding protein -
KONIH1_RS18525 3965199..3966113 - 915 WP_042945598.1 LysR family transcriptional regulator -
KONIH1_RS18530 3966214..3967212 + 999 WP_014229982.1 aldo/keto reductase -
KONIH1_RS18535 3967250..3968176 - 927 WP_042945600.1 LysR family transcriptional regulator -
KONIH1_RS18540 3968353..3968961 + 609 WP_162763184.1 glutathione S-transferase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11740.57 Da        Isoelectric Point: 10.1863

>T47456 WP_014229979.1 NZ_CP008788:c3963959-3963627 [Klebsiella oxytoca KONIH1]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVSLEGAGTKTTGVIRCDQPRTI
DMAARNGKRLESIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T47456 NZ_CP008788:c3963959-3963627 [Klebsiella oxytoca KONIH1]
ATGGACAGAGGGGAAATCTGGCTCGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGCGGAAAACGTCCGGTGCTTAT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGTTACCCGTTGTTGTTCCCATCACCAGCGGTGGAAATTTTGCTCGCA
CGGCAGGTTTCACCGTTTCACTGGAAGGGGCAGGGACAAAAACAACGGGCGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGCTGCCCGGAACGGCAAGCGGCTGGAAAGCATACCAGACGCTGTGGTAAATGAAGTGCTTGCCAGGCTGGATGC
AATTTTAAGTTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9422.88 Da        Isoelectric Point: 4.4607

>AT47456 WP_004852307.1 NZ_CP008788:c3964217-3963960 [Klebsiella oxytoca KONIH1]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT47456 NZ_CP008788:c3964217-3963960 [Klebsiella oxytoca KONIH1]
ATGTATACCACTCGCCTGAAAAAGGTCGGCGGATCCATCATGTTGGCGGTTCCTCCCGCCGTGCTCAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTCGGCATGACCATTAATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCTCATTATTCTC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCTCCTGCGGTG
GGCAAGGAGATCCTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3HDW1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HDW8

References