Detailed information of TA system TA047331

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1811191..1811830
• Replicon: chromosome
• Accession: NZ_CP008740
• Organism: Haemophilus influenzae 2019

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4QJX7
• Locus tag: C645_RS09210
• Protein ID: WP_005650215.1
• Coordinates: 1811525..1811830 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C645_RS09205
• Protein ID: WP_005650217.1
• Coordinates: 1811191..1811514 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C645_RS10365	1806810..1806983	-	174	WP_005657572.1	DUF5363 domain-containing protein	-
C645_RS09190	1806980..1808950	-	1971	WP_046067847.1	tRNA(Met) cytidine acetyltransferase TmcA	-
C645_RS09195	1808955..1809296	-	342	WP_005657576.1	SirB2 family protein	-
C645_RS09200	1809358..1811028	-	1671	WP_005657578.1	energy-dependent translational throttle protein EttA	-
C645_RS09205	1811191..1811514	-	324	WP_005650217.1	HigA family addiction module antidote protein	Antitoxin
C645_RS09210	1811525..1811830	-	306	WP_005650215.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C645_RS09215	1812155..1812775	+	621	WP_005657580.1	zinc transporter binding subunit ZevA	-
C645_RS09220	1812778..1813746	+	969	WP_005657582.1	zinc transporter permease subunit ZevB	-
C645_RS09230	1814362..1816401	+	2040	WP_046067848.1	excinuclease ABC subunit B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12301.93 Da
• Isoelectric Point: 9.0853

>T47331 WP_005650215.1 NZ_CP008740:c1811830-1811525 [Haemophilus influenzae 2019]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T47331 NZ_CP008740:c1811830-1811525 [Haemophilus influenzae 2019]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCCATTCGAGTGAATAAACAATATCGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT47331 WP_005650217.1 NZ_CP008740:c1811514-1811191 [Haemophilus influenzae 2019]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT47331 NZ_CP008740:c1811514-1811191 [Haemophilus influenzae 2019]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACGCTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A806EC70