Detailed information of TA system TA047022

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 1130935..1131467
• Replicon: chromosome
• Accession: NZ_CP007787
• Organism: Burkholderia cepacia strain DDS 7H-2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: B4EDC8
• Locus tag: DM42_RS24475
• Protein ID: WP_006484901.1
• Coordinates: 1131144..1131467 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: B4EDC7
• Locus tag: DM42_RS24470
• Protein ID: WP_012492929.1
• Coordinates: 1130935..1131147 (+)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DM42_RS24435	1126389..1127684	+	1296	WP_034200957.1	Tol-Pal system protein TolB	-
DM42_RS24440	1127702..1128214	+	513	WP_006484980.1	peptidoglycan-associated lipoprotein Pal	-
DM42_RS24445	1128234..1128983	+	750	WP_006484965.1	tol-pal system protein YbgF	-
DM42_RS24455	1129411..1129731	-	321	WP_012492928.1	helix-turn-helix domain-containing protein	-
DM42_RS24460	1129870..1130265	+	396	WP_034200958.1	hypothetical protein	-
DM42_RS24465	1130333..1130785	+	453	WP_006484947.1	hypothetical protein	-
DM42_RS24470	1130935..1131147	+	213	WP_012492929.1	antitoxin MazE family protein	Antitoxin
DM42_RS24475	1131144..1131467	+	324	WP_006484901.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DM42_RS38345	1131787..1132311	+	525	WP_080747365.1	hypothetical protein	-
DM42_RS38350	1132313..1133338	+	1026	WP_080435191.1	relaxase/mobilization nuclease domain-containing protein	-
DM42_RS24485	1133461..1133904	-	444	WP_011544671.1	YiaA/YiaB family protein	-
DM42_RS24490	1134008..1134955	-	948	WP_034200960.1	AraC family transcriptional regulator	-
DM42_RS24495	1135085..1135414	+	330	WP_006484989.1	carboxymuconolactone decarboxylase family protein	-
DM42_RS24505	1135615..1136448	+	834	WP_034200961.1	class II glutamine amidotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (7-67)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11652.77 Da
• Isoelectric Point: 8.9366

>T47022 WP_006484901.1 NZ_CP007787:1131144-1131467 [Burkholderia cepacia]
MNRGSIVTVALQGDFGKARPALVVQSDLFAEHPSVTLLLMSSAMVDAPLIRITVVPSEQNGLRMPSQIAVDKMFTVRREK
IGRPIGHLEDELMVAVNRSLLVFLGLA

Nucleotide

• Length: 324 bp

>T47022 NZ_CP007787:1131144-1131467 [Burkholderia cepacia]
ATGAATCGCGGGAGCATTGTCACAGTCGCGCTCCAAGGTGACTTCGGCAAGGCTCGGCCGGCGCTCGTGGTGCAGTCGGA
CCTGTTTGCCGAACATCCCAGCGTAACGTTGCTGCTAATGTCGAGCGCAATGGTCGATGCGCCCTTGATACGCATTACTG
TTGTCCCATCCGAGCAGAACGGCTTGCGCATGCCGTCCCAGATTGCCGTAGACAAAATGTTCACCGTGCGCCGCGAGAAA
ATCGGTCGCCCCATCGGCCACCTCGAAGATGAACTGATGGTTGCCGTCAATCGTTCGCTACTCGTTTTTCTAGGACTAGC
GTAA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 8025.99 Da
• Isoelectric Point: 5.5492

>AT47022 WP_012492929.1 NZ_CP007787:1130935-1131147 [Burkholderia cepacia]
MAATTSERVRIHRENLRAAGLRPIQIWVPDVRQPGFADECARQSRMVHQSIDENDLLDFIEQVTDLGHSQ

Nucleotide

• Length: 213 bp

>AT47022 NZ_CP007787:1130935-1131147 [Burkholderia cepacia]
ATGGCAGCTACCACTTCCGAGCGTGTTCGAATTCACCGCGAGAATCTTCGTGCGGCCGGGCTACGTCCAATTCAGATATG
GGTGCCGGACGTCCGCCAGCCGGGTTTCGCCGATGAATGCGCTCGGCAATCGCGCATGGTCCACCAGAGCATCGACGAGA
ACGATCTGCTCGATTTCATTGAACAGGTTACGGACCTCGGACACTCACAATGA

Structures

Toxin

• AlphaFold DB: B4EDC8

Antitoxin

• AlphaFold DB: B4EDC7