Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vagCD/VapC-VagC |
| Location | 35984..36627 | Replicon | plasmid pKpQIL-6e6 |
| Accession | NZ_CP007730 | ||
| Organism | Klebsiella pneumoniae subsp. pneumoniae KPNIH10 | ||
Toxin (Protein)
| Gene name | vagD | Uniprot ID | D8L2J9 |
| Locus tag | KPNIH10_RS28890 | Protein ID | WP_001044770.1 |
| Coordinates | 35984..36400 (-) | Length | 139 a.a. |
Antitoxin (Protein)
| Gene name | vagC | Uniprot ID | D5KTK7 |
| Locus tag | KPNIH10_RS28895 | Protein ID | WP_001261282.1 |
| Coordinates | 36397..36627 (-) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KPNIH10_RS28850 | 31526..31762 | - | 237 | WP_000993386.1 | broad-spectrum mercury transporter MerE | - |
| KPNIH10_RS28855 | 31759..32121 | - | 363 | WP_001277456.1 | mercury resistance co-regulator MerD | - |
| KPNIH10_RS28860 | 32139..33833 | - | 1695 | WP_000105636.1 | mercury(II) reductase | - |
| KPNIH10_RS28865 | 33885..34307 | - | 423 | WP_001340589.1 | organomercurial transporter MerC | - |
| KPNIH10_RS28870 | 34343..34453 | - | 111 | Protein_37 | mercuric transport protein periplasmic component | - |
| KPNIH10_RS28875 | 34469..35166 | - | 698 | Protein_38 | IS1 family transposase | - |
| KPNIH10_RS28885 | 35200..35910 | - | 711 | WP_004152334.1 | ATP-binding protein | - |
| KPNIH10_RS28890 | 35984..36400 | - | 417 | WP_001044770.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| KPNIH10_RS28895 | 36397..36627 | - | 231 | WP_001261282.1 | AbrB/MazE/SpoVT family DNA-binding domain-containing protein | Antitoxin |
| KPNIH10_RS33185 | 36584..37045 | + | 462 | WP_072093212.1 | hypothetical protein | - |
| KPNIH10_RS28910 | 37532..37840 | + | 309 | WP_004152336.1 | hypothetical protein | - |
| KPNIH10_RS28915 | 37868..38197 | + | 330 | WP_004152337.1 | hypothetical protein | - |
| KPNIH10_RS28920 | 38223..38621 | + | 399 | WP_004171440.1 | hypothetical protein | - |
| KPNIH10_RS28925 | 38628..38960 | + | 333 | WP_004152339.1 | hypothetical protein | - |
| KPNIH10_RS28930 | 38960..39742 | + | 783 | WP_004152340.1 | site-specific integrase | - |
| KPNIH10_RS30910 | 40634..40864 | - | 231 | WP_011977773.1 | hypothetical protein | - |
| KPNIH10_RS30915 | 40956..41429 | - | 474 | WP_004152341.1 | YkgJ family cysteine cluster protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | blaKPC-3 / blaOXA-9 / blaTEM-1A | - | 1..113639 | 113639 | |
| - | flank | IS/Tn | - | - | 41549..42817 | 1268 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 139 a.a. Molecular weight: 15106.52 Da Isoelectric Point: 7.1084
>T46941 WP_001044770.1 NZ_CP007730:c36400-35984 [Klebsiella pneumoniae subsp. pneumoniae KPNIH10]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR
Download Length: 417 bp
>T46941 NZ_CP007730:c36400-35984 [Klebsiella pneumoniae subsp. pneumoniae KPNIH10]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8756.83 Da Isoelectric Point: 4.4692
>AT46941 WP_001261282.1 NZ_CP007730:c36627-36397 [Klebsiella pneumoniae subsp. pneumoniae KPNIH10]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL
Download Length: 231 bp
>AT46941 NZ_CP007730:c36627-36397 [Klebsiella pneumoniae subsp. pneumoniae KPNIH10]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0H3GYM2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0H3GZG3 |