Detailed information of TA system TA046862

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2153858..2154387
• Replicon: chromosome
• Accession: NZ_CP007674
• Organism: Staphylococcus aureus strain CA15

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: EQ90_RS10980
• Protein ID: WP_000621175.1
• Coordinates: 2153858..2154220 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: EQ90_RS10985
• Protein ID: WP_000948331.1
• Coordinates: 2154217..2154387 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQ90_RS10955	2150836..2151606	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
EQ90_RS10960	2151581..2152060	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
EQ90_RS10965	2152062..2152388	-	327	WP_001052491.1	anti-sigma factor antagonist	-
EQ90_RS10970	2152507..2153508	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
EQ90_RS10980	2153858..2154220	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EQ90_RS10985	2154217..2154387	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
EQ90_RS10990	2154472..2155620	-	1149	WP_001281145.1	alanine racemase	-
EQ90_RS10995	2155686..2156045	-	360	WP_000581200.1	holo-ACP synthase	-
EQ90_RS11000	2156049..2156540	-	492	WP_001205910.1	PH domain-containing protein	-
EQ90_RS11005	2156527..2158110	-	1584	WP_001294626.1	PH domain-containing protein	-
EQ90_RS11010	2158103..2158582	-	480	WP_001287088.1	hypothetical protein	-
EQ90_RS11015	2158790..2159350	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T46862 WP_000621175.1 NZ_CP007674:c2154220-2153858 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T46862 NZ_CP007674:c2154220-2153858 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT46862 WP_000948331.1 NZ_CP007674:c2154387-2154217 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT46862 NZ_CP007674:c2154387-2154217 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1