Detailed information of TA system TA046464

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 5647..6222
• Replicon: plasmid p2_50K
• Accession: NZ_CP007450
• Organism: Yersinia enterocolitica LC20

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LC20_RS23270
• Protein ID: WP_025380182.1
• Coordinates: 5647..5976 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: LC20_RS23275
• Protein ID: WP_025380183.1
• Coordinates: 5977..6222 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LC20_RS23265	4727..5422	-	696	WP_025380181.1	IS6 family transposase	-
LC20_RS23270	5647..5976	-	330	WP_025380182.1	endoribonuclease MazF	Toxin
LC20_RS23275	5977..6222	-	246	WP_025380183.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LC20_RS23280	6730..7803	+	1074	WP_025380184.1	hypothetical protein	-
LC20_RS23285	7910..8254	+	345	WP_025380185.1	hypothetical protein	-
LC20_RS23290	8365..9588	-	1224	WP_025378473.1	IS91 family transposase	-
LC20_RS23295	9741..10613	+	873	WP_025380186.1	ParA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pilW	1..49188	49188
	-	flank	IS/Tn	-	-	4727..5422	695

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-103)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11977.95 Da
• Isoelectric Point: 9.5817

>T46464 WP_025380182.1 NZ_CP007450:c5976-5647 [Yersinia enterocolitica LC20]
MKTYVPDAGDIVWLDFDPQAGHEQAGHRPALVLSPAVYNEKVGLMLCCPMTTQIKGYPFEVLIAGTRPSAVLSDQIKSLD
WRKRNATRKGRVTALELAEVRAKSKALIG

Nucleotide

• Length: 330 bp

>T46464 NZ_CP007450:c5976-5647 [Yersinia enterocolitica LC20]
ATGAAAACCTACGTTCCTGACGCTGGCGATATTGTTTGGCTTGATTTTGATCCGCAGGCTGGCCATGAGCAAGCTGGCCA
TCGCCCGGCCCTGGTACTTAGTCCTGCTGTATATAATGAAAAGGTAGGATTGATGTTGTGTTGCCCTATGACCACACAAA
TAAAGGGATATCCGTTTGAAGTGCTCATCGCTGGGACAAGACCAAGTGCTGTTTTATCTGATCAGATAAAAAGTCTGGAT
TGGCGCAAACGAAATGCAACTCGGAAAGGGCGTGTTACGGCATTAGAATTAGCTGAAGTCAGAGCTAAATCTAAGGCGTT
AATTGGGTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8640.00 Da
• Isoelectric Point: 5.5942

>AT46464 WP_025380183.1 NZ_CP007450:c6222-5977 [Yersinia enterocolitica LC20]
MAQAIVKKWGNSLSVRLPVAVTEAASLKIDDTVDIQVQDGRIVLVPVRAKEVSLQSLLASVTPGNIHHEIDFGLPVGNEA
L

Nucleotide

• Length: 246 bp

>AT46464 NZ_CP007450:c6222-5977 [Yersinia enterocolitica LC20]
ATGGCACAAGCAATAGTAAAAAAATGGGGCAATAGTCTTTCTGTGAGATTACCTGTCGCCGTTACGGAAGCGGCTAGTTT
GAAGATTGATGATACGGTAGATATTCAGGTTCAAGATGGCCGCATAGTCCTTGTACCGGTGCGTGCTAAAGAGGTATCTC
TTCAATCCCTCTTAGCCAGTGTAACCCCTGGAAATATTCATCATGAAATAGATTTTGGATTACCAGTAGGAAATGAGGCT
CTTTAA