Detailed information of TA system TA046440

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3339294..3340135
• Replicon: chromosome
• Accession: NZ_CP007442
• Organism: Escherichia coli ACN001

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1M0WJM1
• Locus tag: J444_RS16475
• Protein ID: WP_000854822.1
• Coordinates: 3339294..3339677 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: J444_RS16480
• Protein ID: WP_048943268.1
• Coordinates: 3339767..3340135 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J444_RS16445	3334939..3336780	+	1842	WP_000437375.1	RNA polymerase sigma factor RpoD	-
J444_RS16450	3336859..3337365	-	507	WP_000228937.1	G/U mismatch-specific DNA glycosylase	-
J444_RS16460	3337664..3338509	-	846	WP_001280435.1	DUF4942 domain-containing protein	-
J444_RS16465	3338606..3338803	-	198	WP_000839263.1	DUF957 domain-containing protein	-
J444_RS16470	3338815..3339303	-	489	WP_001177592.1	hypothetical protein	-
J444_RS16475	3339294..3339677	-	384	WP_000854822.1	type IV toxin-antitoxin system toxin CbtA	Toxin
J444_RS16480	3339767..3340135	-	369	WP_048943268.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
J444_RS16485	3340185..3340829	-	645	WP_029396485.1	hypothetical protein	-
J444_RS16490	3340844..3341065	-	222	WP_000692353.1	DUF987 domain-containing protein	-
J444_RS16495	3341134..3341610	-	477	WP_001384029.1	RadC family protein	-
J444_RS16500	3341625..3342110	-	486	WP_000214317.1	antirestriction protein	-
J444_RS16505	3342201..3343019	-	819	WP_048943269.1	DUF945 domain-containing protein	-
J444_RS27420	3343113..3343291	+	179	Protein_3145	hypothetical protein	-
J444_RS16515	3343430..3343843	-	414	WP_000789536.1	hypothetical protein	-
J444_RS16520	3344113..3344652	-	540	WP_001104026.1	DUF4339 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14295.32 Da
• Isoelectric Point: 8.2830

>T46440 WP_000854822.1 NZ_CP007442:c3339677-3339294 [Escherichia coli ACN001]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHQESKRCN

Nucleotide

• Length: 384 bp

>T46440 NZ_CP007442:c3339677-3339294 [Escherichia coli ACN001]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCAGGAGTCGAAACGATGCAACTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13645.39 Da
• Isoelectric Point: 6.4758

>AT46440 WP_048943268.1 NZ_CP007442:c3340135-3339767 [Escherichia coli ACN001]
VSDTLSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLTDRAGIRGRFSDADANHLDQAFPLLMKQLELMLTSS
ELNPHRQNTVTLYVKGLTCHADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT46440 NZ_CP007442:c3340135-3339767 [Escherichia coli ACN001]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGTACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTACAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCGGATGCGAACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCAGT
GAACTGAATCCCCACCGTCAGAATACCGTCACGCTGTATGTTAAAGGGCTGACCTGCCATGCGGACACCCTCGGCAGCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A1M0WJM1