Detailed information of TA system TA046414

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 1145576..1146231
• Replicon: chromosome
• Accession: NZ_CP007410
• Organism: Pseudomonas brassicacearum strain DF41

Toxin (Protein)

• Gene name: higB
• Uniprot ID: W8Q816
• Locus tag: CD58_RS04820
• Protein ID: WP_025211929.1
• Coordinates: 1145576..1145920 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CD58_RS04825
• Protein ID: WP_025211930.1
• Coordinates: 1145917..1146231 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CD58_RS04795	1140589..1141536	+	948	WP_025211924.1	ubiquinol oxidase subunit II	-
CD58_RS04800	1141540..1143570	+	2031	WP_025211925.1	cytochrome o ubiquinol oxidase subunit I	-
CD58_RS04805	1143574..1144200	+	627	WP_025211926.1	cytochrome o ubiquinol oxidase subunit III	-
CD58_RS04810	1144201..1144536	+	336	WP_025211927.1	cytochrome o ubiquinol oxidase subunit IV	-
CD58_RS04815	1144548..1145435	+	888	WP_025211928.1	heme o synthase	-
CD58_RS04820	1145576..1145920	+	345	WP_025211929.1	toxin	Toxin
CD58_RS04825	1145917..1146231	+	315	WP_025211930.1	helix-turn-helix domain-containing protein	Antitoxin
CD58_RS04830	1146425..1147129	-	705	WP_025211931.1	hypothetical protein	-
CD58_RS04835	1147269..1148012	-	744	WP_025211932.1	DUF72 domain-containing protein	-
CD58_RS04840	1148105..1149118	-	1014	WP_025211933.1	diguanylate cyclase	-
CD58_RS04845	1149240..1150157	-	918	WP_025211934.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-88)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13651.67 Da
• Isoelectric Point: 9.6385

>T46414 WP_025211929.1 NZ_CP007410:1145576-1145920 [Pseudomonas brassicacearum]
MRTLFFETTTFTATVGHYLTDDEYRLLQSYMLQHPEVGDVMPRTGGFRKLRWFDERRGKGKRGGLRVIYYWLMNDRQFWM
FAIYGKDELENLTSEQEKTLKRAIEAELKVRGTL

Nucleotide

• Length: 345 bp

>T46414 NZ_CP007410:1145576-1145920 [Pseudomonas brassicacearum]
ATGCGTACCCTATTTTTTGAAACGACTACGTTCACCGCCACGGTTGGCCATTACCTGACTGACGATGAATATCGCCTGCT
TCAGTCCTACATGCTGCAGCATCCAGAGGTTGGTGACGTCATGCCACGAACCGGCGGCTTTCGTAAGCTGCGCTGGTTCG
ATGAACGTCGTGGCAAAGGGAAGCGAGGTGGGTTGCGAGTCATTTACTATTGGCTCATGAATGATCGTCAGTTCTGGATG
TTTGCGATTTATGGCAAGGACGAGTTGGAAAACCTGACCTCCGAGCAAGAGAAGACGCTCAAGCGCGCCATAGAAGCTGA
GTTGAAAGTGCGAGGTACCTTATGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11808.78 Da
• Isoelectric Point: 9.8898

>AT46414 WP_025211930.1 NZ_CP007410:1145917-1146231 [Pseudomonas brassicacearum]
MKKRDLFAEMMHGVEEMAAHREGKITLRQISIEDKPVPEVSAQEIVALRDKLHMSQAVFAKSIRTSPGTLRNWEQEKSKP
NAQAALLIRLVEKFPDMVERLGAV

Nucleotide

• Length: 315 bp

>AT46414 NZ_CP007410:1145917-1146231 [Pseudomonas brassicacearum]
ATGAAAAAACGTGATCTGTTTGCCGAGATGATGCACGGCGTCGAGGAGATGGCCGCTCACCGTGAGGGCAAGATTACCCT
TCGCCAGATATCGATCGAAGACAAACCGGTTCCCGAGGTGTCGGCGCAAGAAATCGTAGCGTTGCGCGACAAGCTCCATA
TGTCTCAGGCCGTTTTTGCCAAGAGCATCAGGACCAGTCCTGGCACCCTTAGGAACTGGGAGCAGGAGAAGTCCAAACCC
AATGCCCAGGCGGCTCTTCTGATCAGGCTGGTCGAGAAGTTCCCAGACATGGTTGAGCGGCTCGGAGCGGTTTGA

Structures

Toxin

• AlphaFold DB: W8Q816