Detailed information of TA system TA046256

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1197563..1197788
• Replicon: chromosome
• Accession: NZ_CP007275
• Organism: Escherichia coli strain O18

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: NMECO18_RS06465
• Protein ID: WP_000813254.1
• Coordinates: 1197563..1197718 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1197730..1197788 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NMECO18_RS06410	1192643..1192957	-	315	WP_000193273.1	YdfR family protein	-
NMECO18_RS06415	1192962..1193177	-	216	WP_000839572.1	class II holin family protein	-
NMECO18_RS06440	1193902..1194615	-	714	WP_000342820.1	hypothetical protein	-
NMECO18_RS06445	1194861..1195682	-	822	WP_001235237.1	antitermination protein	-
NMECO18_RS06450	1195679..1196053	-	375	WP_001217427.1	RusA family crossover junction endodeoxyribonuclease	-
NMECO18_RS06455	1196066..1197115	-	1050	WP_001265292.1	DUF968 domain-containing protein	-
NMECO18_RS25445	1197117..1197395	-	279	WP_024193993.1	hypothetical protein	-
NMECO18_RS06465	1197563..1197718	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	1197730..1197788	+	59	-	-	Antitoxin
NMECO18_RS06470	1197956..1198621	-	666	WP_000150294.1	epoxyqueuosine reductase QueH	-
NMECO18_RS06475	1198796..1199221	-	426	WP_001151152.1	DUF977 family protein	-
NMECO18_RS06480	1199262..1200332	-	1071	WP_001262357.1	hypothetical protein	-
NMECO18_RS06485	1200404..1200829	-	426	WP_000693918.1	Rha family transcriptional regulator	-
NMECO18_RS06490	1200813..1201085	-	273	WP_000887447.1	helix-turn-helix domain-containing protein	-
NMECO18_RS06495	1201195..1201596	+	402	WP_001329851.1	helix-turn-helix domain-containing protein	-
NMECO18_RS06500	1201624..1201815	+	192	WP_000100899.1	hypothetical protein	-
NMECO18_RS06505	1201815..1202102	+	288	WP_000936799.1	type II toxin-antitoxin system RelE/ParE family toxin	-
NMECO18_RS06510	1202373..1202525	+	153	WP_000379609.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ybtS / ybtX / ybtQ / ybtP	1170476..1217739	47263

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T46256 WP_000813254.1 NZ_CP007275:c1197718-1197563 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T46256 NZ_CP007275:c1197718-1197563 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT46256 NZ_CP007275:1197730-1197788 [Escherichia coli]
CCTTGCCTTTCGGCATGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin