Detailed information of TA system TA046100

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 76210..76853
• Replicon: plasmid pO145-12581
• Accession: NZ_CP007138
• Organism: Escherichia coli O145:H28 str. RM12581

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: B1LRW4
• Locus tag: ECRM12581_RS29455
• Protein ID: WP_001044768.1
• Coordinates: 76210..76626 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D2WFK3
• Locus tag: ECRM12581_RS29460
• Protein ID: WP_001261287.1
• Coordinates: 76623..76853 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECRM12581_RS29425	71372..72046	-	675	WP_001341423.1	IS66 family insertion sequence hypothetical protein	-
ECRM12581_RS29430	72100..72486	-	387	WP_001066949.1	tyrosine-type recombinase/integrase	-
ECRM12581_RS29435	72614..73474	+	861	WP_000704534.1	alpha/beta hydrolase	-
ECRM12581_RS34740	73963..74238	+	276	WP_157776129.1	hypothetical protein	-
ECRM12581_RS29450	75503..76048	+	546	WP_001165114.1	hypothetical protein	-
ECRM12581_RS29455	76210..76626	-	417	WP_001044768.1	type II toxin-antitoxin system VapC family toxin	Toxin
ECRM12581_RS29460	76623..76853	-	231	WP_001261287.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ECRM12581_RS29465	77413..77826	+	414	WP_000465041.1	hypothetical protein	-
ECRM12581_RS29470	77828..78610	+	783	WP_001164205.1	site-specific integrase	-
ECRM12581_RS29475	78783..79136	+	354	WP_000864810.1	colicin M immunity protein	-
ECRM12581_RS34080	79144..79335	-	192	Protein_82	lipid II-degrading bacteriocin	-
ECRM12581_RS34085	79339..79482	-	144	Protein_83	transcriptional regulator	-
ECRM12581_RS29485	79549..80988	-	1440	WP_001213545.1	enterohemolysin T1SS ABC transporter subunit EhxD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	espP / toxB / hlyD / hlyB / hlyA / hlyC	1..87120	87120

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15197.66 Da
• Isoelectric Point: 7.7805

>T46100 WP_001044768.1 NZ_CP007138:c76626-76210 [Escherichia coli O145:H28 str. RM12581]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Nucleotide

• Length: 417 bp

>T46100 NZ_CP007138:c76626-76210 [Escherichia coli O145:H28 str. RM12581]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGCATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8815.90 Da
• Isoelectric Point: 4.3775

>AT46100 WP_001261287.1 NZ_CP007138:c76853-76623 [Escherichia coli O145:H28 str. RM12581]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT46100 NZ_CP007138:c76853-76623 [Escherichia coli O145:H28 str. RM12581]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A606Q844

Antitoxin

• AlphaFold DB: A0A7U9QFC4