Detailed information of TA system TA046099

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 49572..50229
• Replicon: plasmid pRM12581
• Accession: NZ_CP007137
• Organism: Escherichia coli O145:H28 str. RM12581

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: ECRM12581_RS28860
• Protein ID: WP_000270043.1
• Coordinates: 49572..49922 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ECRM12581_RS28865
• Protein ID: WP_000124640.1
• Coordinates: 49927..50229 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECRM12581_RS28810	44641..44820	-	180	Protein_55	helix-turn-helix transcriptional regulator	-
ECRM12581_RS28815	45127..45831	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
ECRM12581_RS28820	46046..46543	-	498	WP_000062185.1	hypothetical protein	-
ECRM12581_RS28825	46546..47034	-	489	WP_001273096.1	DUF1643 domain-containing protein	-
ECRM12581_RS28830	47131..47466	+	336	WP_000683476.1	hypothetical protein	-
ECRM12581_RS28835	47481..47951	-	471	WP_001281821.1	hypothetical protein	-
ECRM12581_RS33340	47944..48315	-	372	WP_000516916.1	hypothetical protein	-
ECRM12581_RS33345	48326..48520	-	195	WP_000343597.1	hypothetical protein	-
ECRM12581_RS28855	48861..49409	-	549	WP_001061574.1	transcriptional regulator	-
ECRM12581_RS28860	49572..49922	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ECRM12581_RS28865	49927..50229	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
ECRM12581_RS28870	50256..50549	-	294	WP_001239997.1	chromosome segregation protein ParM	-
ECRM12581_RS28875	50637..50909	-	273	WP_001043047.1	HU family DNA-binding protein	-
ECRM12581_RS28880	50967..51494	-	528	WP_001236377.1	thermonuclease family protein	-
ECRM12581_RS28890	51725..52582	-	858	WP_001167032.1	hypothetical protein	-
ECRM12581_RS28895	52569..52799	-	231	WP_000972663.1	hypothetical protein	-
ECRM12581_RS28900	52799..53317	-	519	WP_000210756.1	nitrite reductase	-
ECRM12581_RS28905	53314..53760	-	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
ECRM12581_RS28910	53760..54119	-	360	WP_000422768.1	hypothetical protein	-
ECRM12581_RS28915	54176..54604	-	429	WP_000591074.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR	-	1..64562	64562

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T46099 WP_000270043.1 NZ_CP007137:49572-49922 [Escherichia coli O145:H28 str. RM12581]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T46099 NZ_CP007137:49572-49922 [Escherichia coli O145:H28 str. RM12581]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT46099 WP_000124640.1 NZ_CP007137:49927-50229 [Escherichia coli O145:H28 str. RM12581]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT46099 NZ_CP007137:49927-50229 [Escherichia coli O145:H28 str. RM12581]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9