Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3946194..3946921 Replicon chromosome
Accession NZ_CP007136
Organism Escherichia coli O145:H28 str. RM12581

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag ECRM12581_RS20205 Protein ID WP_000550189.1
Coordinates 3946607..3946921 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag ECRM12581_RS20200 Protein ID WP_025404459.1
Coordinates 3946194..3946610 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECRM12581_RS20190 3942283..3943827 + 1545 WP_000258466.1 hypothetical protein -
ECRM12581_RS20195 3944131..3946149 + 2019 WP_000121443.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
ECRM12581_RS20200 3946194..3946610 - 417 WP_025404459.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
ECRM12581_RS20205 3946607..3946921 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
ECRM12581_RS20215 3947206..3948342 - 1137 WP_000018698.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
ECRM12581_RS20220 3948427..3948930 + 504 WP_001295542.1 M48 family metallopeptidase -
ECRM12581_RS20225 3949007..3949699 + 693 WP_000942541.1 vancomycin high temperature exclusion protein -
ECRM12581_RS20230 3949778..3950764 + 987 WP_024221603.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T46093 WP_000550189.1 NZ_CP007136:c3946921-3946607 [Escherichia coli O145:H28 str. RM12581]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T46093 NZ_CP007136:c3946921-3946607 [Escherichia coli O145:H28 str. RM12581]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTAGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15021.50 Da        Isoelectric Point: 4.4547

>AT46093 WP_025404459.1 NZ_CP007136:c3946610-3946194 [Escherichia coli O145:H28 str. RM12581]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEELAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT46093 NZ_CP007136:c3946610-3946194 [Escherichia coli O145:H28 str. RM12581]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATTTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATTAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References