45622

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) stbDE/ParE-YafN
Location 240160..240682 Replicon plasmid p1
Accession NZ_CP006799
Organism Klebsiella pneumoniae subsp. pneumoniae PittNDM01

Toxin (Protein)


Gene name stbE Uniprot ID A0A0C7KG00
Locus tag Q770_RS27400 Protein ID WP_038991638.1
Coordinates 240160..240444 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name stbD Uniprot ID A0A2J4R0U8
Locus tag Q770_RS27405 Protein ID WP_004181777.1
Coordinates 240434..240682 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Q770_RS32005 235360..236455 + 1096 Protein_249 conjugal transfer protein TraG N-terminal domain-containing protein -
Q770_RS27385 236554..237741 - 1188 WP_043907031.1 transposase -
Q770_RS30255 237777..238205 + 429 WP_077256229.1 IS200/IS605 family transposase -
Q770_RS30260 238284..238508 + 225 Protein_252 transposase -
Q770_RS27395 238780..240006 - 1227 WP_040120327.1 transposase -
Q770_RS32010 240042..240143 + 102 Protein_254 IS200/IS605 family transposase -
Q770_RS27400 240160..240444 - 285 WP_038991638.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
Q770_RS27405 240434..240682 - 249 WP_004181777.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
Q770_RS27410 240973..242772 + 1800 WP_043907032.1 ATP-dependent helicase -
Q770_RS27415 243106..244092 + 987 WP_040120328.1 hypothetical protein -
Q770_RS27420 244237..244590 + 354 WP_004181774.1 hypothetical protein -
Q770_RS27425 244652..245473 + 822 WP_004181772.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3')-VI / blaNDM-1 / qnrB1 / aac(6')-Ib-cr / blaOXA-1 / dfrA14 / dfrA12 / aadA2 / qacE / sul1 / armA / msr(E) / mph(E) - 1..283371 283371


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-84)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10993.73 Da        Isoelectric Point: 10.6516

>T45622 WP_038991638.1 NZ_CP006799:c240444-240160 [Klebsiella pneumoniae subsp. pneumoniae PittNDM01]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYHLTKHRN

Download         Length: 285 bp

>T45622 NZ_CP006799:c240444-240160 [Klebsiella pneumoniae subsp. pneumoniae PittNDM01]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACCATTTAACCAAACACAGAAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8925.17 Da        Isoelectric Point: 3.8209

>AT45622 WP_004181777.1 NZ_CP006799:c240682-240434 [Klebsiella pneumoniae subsp. pneumoniae PittNDM01]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Download         Length: 249 bp

>AT45622 NZ_CP006799:c240682-240434 [Klebsiella pneumoniae subsp. pneumoniae PittNDM01]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCGGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGTTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C7KG00

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4R0U8

References