Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-RelB
Location 1028040..1028595 Replicon chromosome
Accession NZ_CP006716
Organism Bifidobacterium breve S27

Toxin (Protein)


Gene name mazF Uniprot ID B7GSS5
Locus tag BS27_RS04210 Protein ID WP_003833460.1
Coordinates 1028040..1028363 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S2ZDC4
Locus tag BS27_RS04215 Protein ID WP_003829112.1
Coordinates 1028350..1028595 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BS27_RS04185 1023068..1023366 - 299 Protein_819 IS256 family transposase -
BS27_RS04190 1023836..1025101 - 1266 WP_025341700.1 Fic family protein -
BS27_RS04195 1025643..1026008 - 366 WP_013140585.1 YccF domain-containing protein -
BS27_RS04200 1026246..1027115 + 870 WP_003829104.1 class I SAM-dependent methyltransferase -
BS27_RS09970 1027209..1027823 + 615 WP_014483846.1 cupin domain-containing protein -
BS27_RS04210 1028040..1028363 - 324 WP_003833460.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BS27_RS04215 1028350..1028595 - 246 WP_003829112.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
BS27_RS04220 1028769..1030025 + 1257 WP_003829116.1 HipA domain-containing protein -
BS27_RS04225 1030290..1030481 + 192 WP_025341701.1 XRE family transcriptional regulator -
BS27_RS04230 1030665..1031936 - 1272 WP_025341702.1 MFS transporter -
BS27_RS04235 1032093..1032755 - 663 WP_025341703.1 TetR/AcrR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1018697..1055887 37190


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-106)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-56)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11829.64 Da        Isoelectric Point: 6.2932

>T45452 WP_003833460.1 NZ_CP006716:c1028363-1028040 [Bifidobacterium breve S27]
MKRGEIRTLQADGYASKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRVLGLL

Download         Length: 324 bp

>T45452 NZ_CP006716:c1028363-1028040 [Bifidobacterium breve S27]
ATGAAACGAGGTGAGATTCGGACGTTGCAGGCCGATGGCTACGCCAGCAAGCCACGGCCGGTTGTCATCGTGCAGAGCGA
TGCCGTCGATCGGTTCGATTCCGTCATCACTTGTCTGCTGACCTCATACGATTCCTCCGACATCGACACGCGGGTCCGAT
TGGAACCAAGTCCGGAGAACGGCCTGAACAAAGTGAGTTACGTGATGACGGACAAGATCGTCACCGTCGACCGCAAACTG
CTGGGATACCAAGTCGGCGTGGTTGATGATGCAGCCATGGCGAATATCGGCCGTCAGCTCATGCGGGTTCTAGGTTTGCT
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9339.43 Da        Isoelectric Point: 4.5881

>AT45452 WP_003829112.1 NZ_CP006716:c1028595-1028350 [Bifidobacterium breve S27]
MSMQIATRVDDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDET
R

Download         Length: 246 bp

>AT45452 NZ_CP006716:c1028595-1028350 [Bifidobacterium breve S27]
ATGAGCATGCAGATTGCCACGCGAGTTGATGACGAGCAGGCTGCGTTGTTTAAGGAGACGACCCGACAGCTGGGTACCAC
CCCGGCCGATGCCCTGCGTATGTTCATCAGCGCGTTCAATGATTACCGTGGGTTCCCCTATGAGGTGCGTTTGCCACGAA
ATGACGTGGAGCCGTTTGCGTCCGAGCGGGACGCTACTGAGTATGCCAGTCGTCTCGCCTTGAGGATGTCCGATGAAACG
AGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4R0SL31

Antitoxin

Source ID Structure
AlphaFold DB A0A087B6Q6

References