Detailed information of TA system TA045446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-RelB
• Location: 1289664..1290230
• Replicon: chromosome
• Accession: NZ_CP006713
• Organism: Bifidobacterium breve JCM 7019

Toxin (Protein)

• Gene name: relE
• Uniprot ID: W6EX94
• Locus tag: B7019_RS05690
• Protein ID: WP_003831807.1
• Coordinates: 1289937..1290230 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: B7019_RS05685
• Protein ID: WP_025221722.1
• Coordinates: 1289664..1289933 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7019_RS10705	1285225..1285386	+	162	Protein_1106	MFS transporter	-
B7019_RS05660	1285571..1286584	-	1014	WP_025221717.1	VanZ family protein	-
B7019_RS05665	1286731..1287555	+	825	WP_025221718.1	aminotransferase class V-fold PLP-dependent enzyme	-
B7019_RS10710	1287624..1287923	+	300	WP_025221719.1	DUF2316 family protein	-
B7019_RS05675	1287964..1288497	-	534	WP_025221720.1	GNAT family N-acetyltransferase	-
B7019_RS10715	1288854..1288973	+	120	WP_171843146.1	IS3 family transposase	-
B7019_RS05680	1288982..1289479	+	498	Protein_1112	FtsX-like permease family protein	-
B7019_RS05685	1289664..1289933	+	270	WP_025221722.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
B7019_RS05690	1289937..1290230	+	294	WP_003831807.1	type II toxin-antitoxin system YafQ family toxin	Toxin
B7019_RS11160	1290236..1290742	-	507	WP_025221723.1	hypothetical protein	-
B7019_RS05700	1290741..1291139	+	399	WP_015438839.1	hypothetical protein	-
B7019_RS05705	1291234..1291596	-	363	WP_015438840.1	hypothetical protein	-
B7019_RS05710	1291720..1292595	-	876	WP_025221724.1	EamA family transporter	-
B7019_RS05715	1292799..1293422	-	624	WP_016462919.1	hypothetical protein	-
B7019_RS05720	1293419..1294147	-	729	WP_016462918.1	hypothetical protein	-
B7019_RS05725	1294150..1294806	-	657	WP_025221725.1	ATP-binding cassette domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1287964..1306189	18225

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-95)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (3-88)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11218.98 Da
• Isoelectric Point: 9.7881

>T45446 WP_003831807.1 NZ_CP006713:1289937-1290230 [Bifidobacterium breve JCM 7019]
MLKPDYTAAFQRDIKKLKRKHTDLRPLKEVIRLVLEDTADSKEVLRRRHRAHTLTGDLNGVLECHIGNAGDWLLLWIRDD
GTAMFMRTGSHDELLGK

Nucleotide

• Length: 294 bp

>T45446 NZ_CP006713:1289937-1290230 [Bifidobacterium breve JCM 7019]
ATGCTCAAACCGGATTACACAGCGGCATTCCAGCGAGATATCAAGAAACTCAAACGCAAGCACACTGATTTACGCCCGCT
GAAAGAAGTCATTCGTCTGGTTCTTGAGGACACGGCGGACTCCAAGGAAGTATTGAGGCGTAGGCATCGCGCCCACACAC
TTACCGGTGATTTAAACGGCGTACTGGAATGCCACATCGGCAATGCCGGCGACTGGCTGCTTCTATGGATTCGCGATGAT
GGTACCGCCATGTTCATGCGAACCGGCAGCCATGACGAACTGTTGGGCAAATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9859.22 Da
• Isoelectric Point: 6.3186

>AT45446 WP_025221722.1 NZ_CP006713:1289664-1289933 [Bifidobacterium breve JCM 7019]
VSMASVTVRVDADTKQKAANIAEDLGLDLSSVTRAFYKQIVRERRIPLNLSYTTVPPETMKALDEAKNRLADGKPRFNSA
DEMFDSLGI

Nucleotide

• Length: 270 bp

>AT45446 NZ_CP006713:1289664-1289933 [Bifidobacterium breve JCM 7019]
GTGAGCATGGCATCCGTCACAGTCCGCGTGGATGCGGACACCAAGCAGAAAGCCGCAAACATAGCCGAGGATCTCGGTCT
GGACTTGTCTTCGGTCACCCGCGCCTTCTACAAGCAGATTGTCCGGGAGAGGCGCATTCCTCTAAACCTTTCCTATACGA
CCGTGCCGCCTGAAACCATGAAGGCCCTTGACGAGGCGAAAAACAGGCTCGCCGACGGCAAACCTCGTTTCAACAGTGCC
GATGAGATGTTCGATTCGTTGGGAATCTGA

Structures

Toxin

• AlphaFold DB: W6EX94