Detailed information of TA system TA044564

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-YefM
• Location: 271632..272194
• Replicon: plasmid pIDH5335-1
• Accession: NZ_AP028130
• Organism: Vibrio fluvialis strain IDH05335

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: QUF32_RS24160
• Protein ID: WP_038152854.1
• Coordinates: 271632..271934 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: QUF32_RS24165
• Protein ID: WP_004729600.1
• Coordinates: 271949..272194 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUF32_RS24140 (KKIDH5335_47090)	266692..267834	-	1143	WP_065274108.1	RNA-guided endonuclease TnpB family protein	-
QUF32_RS24145 (KKIDH5335_47100)	267891..268319	+	429	WP_065274109.1	IS200/IS605 family transposase	-
QUF32_RS24150 (KKIDH5335_47110)	269347..270423	+	1077	WP_038152856.1	replication initiation protein	-
QUF32_RS24155 (KKIDH5335_47120)	271033..271635	+	603	WP_004729602.1	recombinase family protein	-
QUF32_RS24160 (KKIDH5335_47130)	271632..271934	-	303	WP_038152854.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QUF32_RS24165 (KKIDH5335_47140)	271949..272194	-	246	WP_004729600.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
QUF32_RS24170 (KKIDH5335_47150)	272307..272843	+	537	Protein_297	recombinase family protein	-
QUF32_RS24175 (KKIDH5335_47160)	273144..274070	-	927	WP_102137167.1	IS5 family transposase	-
QUF32_RS24180 (KKIDH5335_47170)	274182..275210	-	1029	WP_286236284.1	IS630 family transposase	-
QUF32_RS24185 (KKIDH5335_47180)	275270..276348	-	1079	Protein_300	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	ARR-3 / cmlA1 / blaOXA-10 / ant(3'')-Ia / qacE / sul1 / blaOXA-1 / aac(6')-Ib-cr / blaNDM-1 / blaCTX-M-15 / aac(6')-Ib / blaOXA-9 / blaTEM-1A / mph(A) / dfrA1	-	1..370014	370014
	-	inside	IScluster/Tn	-	-	266692..291085	24393

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-89)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-56)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11644.32 Da
• Isoelectric Point: 6.7572

>T44564 WP_038152854.1 NZ_AP028130:c271934-271632 [Vibrio fluvialis]
MSSYKLSPLAQSDLTDIRRYTIEHWGSTQWSVYFNELQESMSLLASNELIGIQIPEMGERYYRFPLKHHVIYYITQEDHI
VIVAVLGKHMSPAKHFASIS

Nucleotide

• Length: 303 bp

>T44564 NZ_AP028130:c271934-271632 [Vibrio fluvialis]
ATGAGCAGTTATAAGCTTTCACCTTTAGCCCAGTCTGATCTCACTGATATTCGTCGTTACACCATAGAACATTGGGGCAG
TACTCAATGGTCCGTCTATTTTAATGAATTACAGGAATCGATGTCATTGCTGGCAAGTAACGAACTGATCGGAATTCAGA
TCCCGGAAATGGGAGAGCGATACTACCGTTTTCCTCTGAAGCACCATGTGATTTATTACATCACTCAGGAAGACCATATC
GTTATCGTCGCTGTGCTGGGTAAGCATATGTCTCCTGCAAAACATTTTGCGTCTATTTCTTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9115.57 Da
• Isoelectric Point: 4.5702

>AT44564 WP_004729600.1 NZ_AP028130:c272194-271949 [Vibrio fluvialis]
MQSLTANTAKTKFGDLLMKVQREPVQISKNGSPVAVMMSCEEYEQLEALKMMLVKSRFEQAEIDIRNGDLVDGDEFMKSL
A

Nucleotide

• Length: 246 bp

>AT44564 NZ_AP028130:c272194-271949 [Vibrio fluvialis]
ATGCAATCATTAACAGCAAATACAGCAAAAACCAAATTTGGCGATTTACTGATGAAGGTTCAGCGTGAACCCGTGCAAAT
CAGTAAAAATGGCTCACCGGTTGCGGTGATGATGTCCTGTGAAGAATATGAGCAATTAGAAGCACTCAAGATGATGCTGG
TTAAATCTCGTTTTGAACAGGCAGAAATTGATATCAGGAATGGCGATCTGGTCGATGGTGATGAATTTATGAAATCTCTG
GCTTAA