Detailed information of TA system TA044523

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4959338..4959933
• Replicon: chromosome
• Accession: NZ_AP028094
• Organism: Escherichia coli strain JNE132847

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: -
• Locus tag: JE76HU_RS24895
• Protein ID: WP_024215840.1
• Coordinates: 4959583..4959933 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: U9Y2K1
• Locus tag: JE76HU_RS24890
• Protein ID: WP_001223208.1
• Coordinates: 4959338..4959589 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JE76HU_RS24880 (4955003)	4955003..4958782	+	3780	WP_137549569.1	autotransporter assembly complex protein TamB	-
JE76HU_RS24885 (4958785)	4958785..4959126	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
JE76HU_RS24890 (4959338)	4959338..4959589	+	252	WP_001223208.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
JE76HU_RS24895 (4959583)	4959583..4959933	+	351	WP_024215840.1	endoribonuclease toxin ChpB	Toxin
JE76HU_RS24900 (4960013)	4960013..4960543	-	531	WP_000055075.1	inorganic diphosphatase	-
JE76HU_RS24905 (4960853)	4960853..4961809	+	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
JE76HU_RS24910 (4961949)	4961949..4963451	+	1503	WP_000205813.1	sugar ABC transporter ATP-binding protein	-
JE76HU_RS24915 (4963465)	4963465..4964487	+	1023	WP_001296689.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12541.47 Da
• Isoelectric Point: 5.6219

>T44523 WP_024215840.1 NZ_AP028094:4959583-4959933 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQSAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T44523 NZ_AP028094:4959583-4959933 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAAG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9271.58 Da
• Isoelectric Point: 4.4201

>AT44523 WP_001223208.1 NZ_AP028094:4959338-4959589 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT44523 NZ_AP028094:4959338-4959589 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LQ26