Detailed information of TA system TA044519

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3791664..3792463
• Replicon: chromosome
• Accession: NZ_AP028094
• Organism: Escherichia coli strain JNE132847

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: JE76HU_RS19295
• Protein ID: WP_024216030.1
• Coordinates: 3791999..3792463 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: JE76HU_RS19290
• Protein ID: WP_001307405.1
• Coordinates: 3791664..3791999 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JE76HU_RS19275 (3787449)	3787449..3788219	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
JE76HU_RS19280 (3788235)	3788235..3789569	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
JE76HU_RS19285 (3789944)	3789944..3791515	+	1572	WP_001273741.1	galactarate dehydratase	-
JE76HU_RS19290 (3791664)	3791664..3791999	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
JE76HU_RS19295 (3791999)	3791999..3792463	+	465	WP_024216030.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
JE76HU_RS19300 (3792518)	3792518..3793327	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
JE76HU_RS19305 (3793576)	3793576..3794856	+	1281	WP_000681920.1	tagatose-bisphosphate aldolase subunit KbaZ	-
JE76HU_RS19310 (3794879)	3794879..3795352	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
JE76HU_RS19315 (3795363)	3795363..3796142	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
JE76HU_RS19320 (3796132)	3796132..3797010	+	879	WP_001380079.1	PTS N-acetylgalactosamine transporter subunit IID	-
JE76HU_RS19325 (3797028)	3797028..3797462	+	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17826.21 Da
• Isoelectric Point: 9.2153

>T44519 WP_024216030.1 NZ_AP028094:3791999-3792463 [Escherichia coli]
MDFPQRVNGWVLYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGLYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESFTQETEENH

Nucleotide

• Length: 465 bp

>T44519 NZ_AP028094:3791999-3792463 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGTGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCTTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCTTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT44519 WP_001307405.1 NZ_AP028094:3791664-3791999 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT44519 NZ_AP028094:3791664-3791999 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0XVC7