Detailed information of TA system TA044497

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 456482..457319
• Replicon: chromosome
• Accession: NZ_AP028094
• Organism: Escherichia coli strain JNE132847

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: JE76HU_RS02070
• Protein ID: WP_000227784.1
• Coordinates: 456482..457024 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: JE76HU_RS02075
• Protein ID: WP_001297137.1
• Coordinates: 457008..457319 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JE76HU_RS02050 (452021)	452021..452932	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
JE76HU_RS02055 (453100)	453100..453591	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
JE76HU_RS02060 (453719)	453719..455083	-	1365	WP_001000978.1	MFS transporter	-
JE76HU_RS02065 (455491)	455491..456426	+	936	WP_001297127.1	tetratricopeptide repeat protein	-
JE76HU_RS02070 (456482)	456482..457024	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
JE76HU_RS02075 (457008)	457008..457319	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
JE76HU_RS02080 (457504)	457504..458394	-	891	WP_000971336.1	heme o synthase	-
JE76HU_RS02085 (458406)	458406..458735	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
JE76HU_RS02090 (458735)	458735..459349	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
JE76HU_RS02095 (459339)	459339..461330	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
JE76HU_RS02100 (461352)	461352..462299	-	948	WP_001239440.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T44497 WP_000227784.1 NZ_AP028094:c457024-456482 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T44497 NZ_AP028094:c457024-456482 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT44497 WP_001297137.1 NZ_AP028094:c457319-457008 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT44497 NZ_AP028094:c457319-457008 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3