Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3825034..3825575 Replicon chromosome
Accession NZ_AP027960
Organism Escherichia coli strain VE-T2

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag QQK13_RS18715 Protein ID WP_000615982.1
Coordinates 3825297..3825575 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag QQK13_RS18710 Protein ID WP_000729704.1
Coordinates 3825034..3825294 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQK13_RS18685 (3820070) 3820070..3821209 + 1140 WP_000785662.1 flagellar type III secretion system protein FlhB -
QQK13_RS18690 (3821193) 3821193..3823286 + 2094 WP_001140806.1 flagellar biosynthesis protein FlhA -
QQK13_RS18695 (3823391) 3823391..3823669 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
QQK13_RS18700 (3823662) 3823662..3824018 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
QQK13_RS18705 (3824075) 3824075..3824848 - 774 WP_000543899.1 C40 family peptidase -
QQK13_RS18710 (3825034) 3825034..3825294 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
QQK13_RS18715 (3825297) 3825297..3825575 + 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
QQK13_RS18720 (3825731) 3825731..3826471 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
QQK13_RS18725 (3826442) 3826442..3827209 - 768 WP_000333380.1 class II glutamine amidotransferase -
QQK13_RS18730 (3827314) 3827314..3827892 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T44291 WP_000615982.1 NZ_AP027960:3825297-3825575 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T44291 NZ_AP027960:3825297-3825575 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT44291 WP_000729704.1 NZ_AP027960:3825034-3825294 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT44291 NZ_AP027960:3825034-3825294 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References