Detailed information of TA system TA044235

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4111123..4111955
• Replicon: chromosome
• Accession: NZ_AP027953
• Organism: Escherichia coli strain VE-F5

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: QQJ99_RS20135
• Protein ID: WP_000854765.1
• Coordinates: 4111123..4111497 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: QQJ99_RS20140
• Protein ID: WP_001295723.1
• Coordinates: 4111587..4111955 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QQJ99_RS20115 (4106580)	4106580..4109696	+	3117	WP_000149665.1	HsdR family type I site-specific deoxyribonuclease	-
QQJ99_RS20120 (4110187)	4110187..4110336	-	150	Protein_3934	hypothetical protein	-
QQJ99_RS20125 (4110442)	4110442..4110618	-	177	WP_000839290.1	DUF957 domain-containing protein	-
QQJ99_RS20130 (4110635)	4110635..4111126	-	492	WP_000976842.1	DUF5983 family protein	-
QQJ99_RS20135 (4111123)	4111123..4111497	-	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
QQJ99_RS20140 (4111587)	4111587..4111955	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
QQJ99_RS20145 (4112118)	4112118..4112339	-	222	WP_088561025.1	DUF987 domain-containing protein	-
QQJ99_RS20150 (4112402)	4112402..4112878	-	477	WP_001186774.1	RadC family protein	-
QQJ99_RS20155 (4112894)	4112894..4113367	-	474	WP_000855059.1	antirestriction protein	-
QQJ99_RS20160 (4113709)	4113709..4114527	-	819	WP_001234729.1	DUF932 domain-containing protein	-
QQJ99_RS20165 (4114645)	4114645..4114840	-	196	Protein_3943	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB	4092691..4125622	32931

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T44235 WP_000854765.1 NZ_AP027953:c4111497-4111123 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T44235 NZ_AP027953:c4111497-4111123 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGACTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT44235 WP_001295723.1 NZ_AP027953:c4111955-4111587 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT44235 NZ_AP027953:c4111955-4111587 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: S1NM52