Detailed information of TA system TA044201

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2996488..2997323
• Replicon: chromosome
• Accession: NZ_AP027946
• Organism: Escherichia coli strain VE-F10

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: QQK65_RS14495
• Protein ID: WP_105075127.1
• Coordinates: 2996488..2996865 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: QQK65_RS14500
• Protein ID: WP_024257643.1
• Coordinates: 2996955..2997323 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QQK65_RS14460 (2991681)	2991681..2993402	+	1722	WP_000543083.1	cysteine/glutathione ABC transporter ATP-binding protein/permease CydC	-
QQK65_RS14465 (2993444)	2993444..2994148	+	705	WP_001241678.1	leucyl/phenylalanyl-tRNA--protein transferase	-
QQK65_RS14470 (2994433)	2994433..2994651	+	219	WP_001040187.1	translation initiation factor IF-1	-
QQK65_RS14480 (2995142)	2995142..2995984	-	843	WP_105075128.1	DUF4942 domain-containing protein	-
QQK65_RS14485 (2996069)	2996069..2996266	-	198	WP_023563519.1	DUF957 domain-containing protein	-
QQK65_RS14490 (2996294)	2996294..2996491	-	198	Protein_2835	DUF5983 family protein	-
QQK65_RS14495 (2996488)	2996488..2996865	-	378	WP_105075127.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
QQK65_RS14500 (2996955)	2996955..2997323	-	369	WP_024257643.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
QQK65_RS14505 (2997403)	2997403..2997624	-	222	WP_000692309.1	DUF987 domain-containing protein	-
QQK65_RS14510 (2997693)	2997693..2998169	-	477	WP_001186725.1	RadC family protein	-
QQK65_RS14515 (2998185)	2998185..2998670	-	486	WP_105075126.1	antirestriction protein	-
QQK65_RS14520 (2998761)	2998761..2999579	-	819	WP_001234702.1	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13943.00 Da
• Isoelectric Point: 8.5163

>T44201 WP_105075127.1 NZ_AP027946:c2996865-2996488 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACIRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T44201 NZ_AP027946:c2996865-2996488 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTATCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGTCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13678.33 Da
• Isoelectric Point: 6.4768

>AT44201 WP_024257643.1 NZ_AP027946:c2997323-2996955 [Escherichia coli]
VSDTFSGTTHPDDNHDRPWWGQPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNSRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT44201 NZ_AP027946:c2997323-2996955 [Escherichia coli]
GTGTCAGACACATTCTCCGGGACAACGCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCAGCCCTGTACCGTGAC
ACCTTGTTTTGGCGCCCGTCTGGTGCAGGAGGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATTCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA