Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4168383..4168933 Replicon chromosome
Accession NZ_AP027926
Organism Escherichia fergusonii strain 30038

Toxin (Protein)


Gene name ccdB Uniprot ID F0JPU1
Locus tag QUE84_RS19995 Protein ID WP_001160963.1
Coordinates 4168383..4168697 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag QUE84_RS20000 Protein ID WP_000125566.1
Coordinates 4168700..4168933 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE84_RS19970 (4164058) 4164058..4165344 + 1287 WP_000800490.1 peptidylprolyl isomerase SurA -
QUE84_RS19975 (4165344) 4165344..4166333 + 990 WP_181668157.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
QUE84_RS19980 (4166330) 4166330..4167151 + 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
QUE84_RS19985 (4167154) 4167154..4167531 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
QUE84_RS19990 (4167538) 4167538..4168386 + 849 WP_223689152.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
QUE84_RS19995 (4168383) 4168383..4168697 - 315 WP_001160963.1 CcdB family protein Toxin
QUE84_RS20000 (4168700) 4168700..4168933 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
QUE84_RS20005 (4169019) 4169019..4169498 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
QUE84_RS20010 (4169691) 4169691..4171553 - 1863 WP_046076788.1 glutathione-regulated potassium-efflux system protein KefC -
QUE84_RS20015 (4171546) 4171546..4172076 - 531 WP_000600701.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
QUE84_RS20020 (4172184) 4172184..4173515 - 1332 WP_001183184.1 MFS transporter -
QUE84_RS20025 (4173574) 4173574..4173861 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11722.70 Da        Isoelectric Point: 8.0666

>T44173 WP_001160963.1 NZ_AP027926:c4168697-4168383 [Escherichia fergusonii]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSHIRPPERLNPILLLVDGKEYVLMTHETATVPVNTLG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T44173 NZ_AP027926:c4168697-4168383 [Escherichia fergusonii]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCATATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACACGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACTTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT44173 WP_000125566.1 NZ_AP027926:c4168933-4168700 [Escherichia fergusonii]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT44173 NZ_AP027926:c4168933-4168700 [Escherichia fergusonii]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB F0JPU1

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References